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Series GSE167600 Query DataSets for GSE167600
Status Public on Feb 25, 2022
Title SCD inhibition reverses immune, synaptic and behavioral deficits in an animal model of Alzheimer’s disease [scRNA-Seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Early- and late-onset forms of Alzheimer’s disease (AD) share common features that include abnormalities in lipid metabolism, immune response and synaptic function. Of these, the role of lipids remains the least understood. Our study revealed that molecular functions related to Stearoyl-CoA Desaturase (SCD), the rate limiting enzyme in monounsaturated fatty acid synthesis were specifically altered in the hippocampus of 3xTg-AD (a model of early-onset AD). Remarkably, infusion of an SCD inhibitor (SCDi) reversed 40% of altered immune and synapse genes, rescue dendritic spine number and structure, recovered activity-associated immediate-early gene expression and restored learning and memory in 3xTg-AD mice. In addition, we employed single cell RNA sequencing to characterize the cellular landscape of microglia subpopulations within the 3xTg hippocampus and uncovered that SCDi reversed microglial activation and polarization. Together, we show that a single lipid enzyme, SCD, impinges on the core features of AD.
Overall design For each treatment group the ipsilateral hippocampi from 4 animals were dissociated into single cells and pooled before staining with CD45 mutiplexing kit from BD Biosciences.
Contributor(s) Hamilton LK, Fernandes KJ, Moquin-Beaudry G
Citation(s) 35443751
Submission date Feb 26, 2021
Last update date May 05, 2022
Contact name Gaël Moquin-Beaudry
Organization name CRCHUM
Street address 900 Saint-Denis street
City Montréal
State/province QC
ZIP/Postal code H2X 0A9
Country Canada
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (1)
GSM5111239 WT DMSO, 3xTg DMSO, WT SCDi, 3xTg SCDi
This SubSeries is part of SuperSeries:
GSE167605 SCD inhibition reverses immune, synaptic and behavioral deficits in an animal model of Alzheimer’s disease
BioProject PRJNA705117
SRA SRP308356

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Supplementary file Size Download File type/resource
GSE167600_RAW.tar 590.9 Mb (http)(custom) TAR (of RDS, TSV)
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