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| Status |
Public on Nov 15, 2023 |
| Title |
Direct Reprogramming of Non-limb Fibroblasts to Cells with Properties of Limb Progenitors [bulkRNA-seq] |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The early limb bud consists of mesenchymal progenitors (limb progenitors) derived from the lateral plate mesoderm (LPM) that produce most of the tissues of the mature limb bud. The LPM also gives rise to the mesodermal components of the trunk, flank and neck. However, the mesenchymal cells generated at these other axial levels cannot produce the variety of cell types found in the limb bud, nor can they be directed to form a patterned appendage-like structure, even when placed in the context of the signals responsible for organizing the limb bud. Here, by taking advantage of a direct reprogramming approach, we find a set of factors (Prdm16, Zbtb16, and Lin28) normally expressed in the early limb bud, that are capable of imparting limb progenitor-like properties to non-limb fibroblasts. Cells reprogrammed by these factors show similar gene expression profiles, and can differentiate into similar cell types, as endogenous limb progenitors. The further addition of Lin41 potentiates proliferation of the reprogrammed cells while suppressing differentiation. These results suggest that these same four key factors may play pivotal roles in the specification of endogenous limb progenitors.
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| Overall design |
RNA-seq of mesenchymal cells between different source tissues (Forelimb, Hindlimb, Neck, Flank)
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| Contributor(s) |
Atsuta Y, Lee C, Rodrigues AR, Colle C, Tomizawa RR, Lujan EG, Tschopp P, Christodoulou DC, Gorham JM, Vannier J, Seidman CE, Seidman JG, Pourquié O, Tabin CJ |
| Citation(s) |
38320485 |
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| Submission date |
Feb 24, 2021 |
| Last update date |
Feb 14, 2024 |
| Contact name |
CHANGHEE LEE |
| E-mail(s) |
chlee@genetics.med.harvard.edu
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| Phone |
617-432-6534
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| Organization name |
Harvard Medical School
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| Department |
Genetics
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| Lab |
Clifford Tabin
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| Street address |
77 AVENUE LOUIS PASTEUR
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| City |
BOSTON |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL19005 |
Illumina HiSeq 2500 (Gallus gallus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE167423 |
Direct Reprogramming of Non-limb Fibroblasts to Cells with Properties of Limb Progenitors |
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| Relations |
| BioProject |
PRJNA704626 |
| SRA |
SRP307945 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE167419_DEGoutput.txt.gz |
4.5 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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