|
| Status |
Public on Jun 28, 2021 |
| Title |
RNA-seq of ΔDS deletion vs +WT Complemented seeds |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We identified a regulator of Arabidopsis thaliana seed germination: FLOE1 (AT4G28300). We used RNA-seq to uncover genes that are differentially regulated in dry seeds, imbibed seeds and seeds imbibed in 220mM NaCl in mutant lines complemented with a ΔDS deletion version of FLOE1 (ΔDS) compared to mutants complemented with a WT version of FLOE1 (+WT).
|
| |
|
| Overall design |
Imbibition and salt: after 5 days of stratification at 4C in the dark on MS media containing either 0 or 220mM NaCl, seeds were transferred to a growth chamber at 22C with normal light conditions for 4hours. RNA was then extracted from these seeds as well as from dry seeds. RNA was then sent to Novogene Corporation Inc. for library preparation (250-300 bp insert cDNA library) and sequencing (2x150 bp paired-end reads on an Illumina Platform). Three replicates were conducted for each genotype (+ΔDS or +WT) and condition (dry, imbibed, salt).
|
| |
|
| Contributor(s) |
Dorone Y |
| Citation(s) |
34233164 |
| |
| Submission date |
Feb 22, 2021 |
| Last update date |
Sep 28, 2021 |
| Contact name |
Yanniv Dorone |
| Organization name |
Stanford University
|
| Street address |
371 Jane Stanford Way
|
| City |
Stanford |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
|
| Samples (18)
|
|
| Relations |
| BioProject |
PRJNA704079 |
| SRA |
SRP307584 |
Less...
More...