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Status |
Public on Aug 31, 2021 |
Title |
Evolutionary rewiring of wheat abiotic stress responsive network by lineage-specific transposable elements I |
Organism |
Triticum urartu |
Experiment type |
Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing Methylation profiling by high throughput sequencing
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Summary |
The yield of wheat is highly impacted by environmental stresses. The combinatorial regulation of sequence-specific transcription factors(TFs) defines a regulatory network that underlies plant stress responses. Here we created a comprehensive catalog of genomic binding sites of 115 TFs underlying abiotic stress responses by leveraging DAP-seq in Triticum Urartu, along with epigenomic profiles. The majority of gene distant TF binding sites(TFBS) are embedded in transposable elements(TEs), whose functional relevance was supported by a signature of purifying selection and active epigenomic features. Furthermore, ~30% non-TE TFBS share high sequence similarity with TE-embeded TFBS, potentially derived from Triticeae-specific TEs and have almost no sequence homology in non-Triticeae species. The expansion of TE-derived TFBS in wheat linked to wheat-specific stress responsive genes, suggesting that TEs are an important driving force for regulatory innovation. Altogether, TEs have significantly and continuously shaped regulatory network in wheat adaptation.
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Overall design |
In order to delineate the major TF regulatory circuitry to abiotic stresses in Triticum Urartu, we performed DAP-seq to obtain a genome-wide binding profile of stress response TFs, ChIP-seq for three well-studied histone modifications (including H3K9ac, H3K4me3 and H3K27me3), DNase-seq, Bisulphite-seq and RNA-seq in 5 stresses.
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Contributor(s) |
Zhang Y |
Citation(s) |
34503979 |
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Submission date |
Feb 22, 2021 |
Last update date |
Nov 30, 2021 |
Contact name |
yijing zhang |
E-mail(s) |
zhangyijing@fudan.edu.cn
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Organization name |
Fudan University
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Department |
Biochemistry
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Lab |
Functional Epigenomics Group
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Street address |
2005 Songhu Road
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City |
shanghai |
ZIP/Postal code |
200438 |
Country |
China |
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Platforms (2) |
GPL29754 |
Illumina NovaSeq 6000 (Triticum urartu) |
GPL29755 |
HiSeq X Ten (Triticum urartu) |
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Samples (25)
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This SubSeries is part of SuperSeries: |
GSE167229 |
Evolutionary rewiring of wheat abiotic stress responsive network by lineage-specific transposable elements |
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Relations |
BioProject |
PRJNA703956 |
SRA |
SRP307514 |