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Series GSE166743 Query DataSets for GSE166743
Status Public on Jul 07, 2022
Title eIF1-eIF4G1 inhibitors uncover alternative translation activation of stress-response genes via enhanced ribosome loading and 5’UTR translation [MARS-seq]
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The Ribo-seq and TI-seq analysis following i14G1s (eI1-eIF4G1 inhibitors) treatments uncover opposing roles of eIF1-eIF4G1 and eIF4E-eIF4G1 in scanning-dependent and independent translation. Furthermore, i14G1s inhibition of eIF4G1-eIF1 resulted in translation activation of ER/UPR stress-response genes via enhanced ribosome loading, elevated 5’UTR translation at near cognate AUGs, and unexpected concomitant upregulation of coding-region translation.
 
Overall design Ribo-seq and TI-seq analysis was performed either in HEK293T cells treated with either DMSO (vehicle control), i14G1-10 or i14G1-12.
 
Contributor(s) Sehrawat U, Haimov O, Harush AT, Plotnikov A, Leshkowitz D, Dikstein R
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Submission date Feb 15, 2021
Last update date Jul 07, 2022
Contact name Dena Leshkowitz
E-mail(s) dena.leshkowitz@weizmann.ac.il
Organization name Weizmann Institute of Science
Department Bioinformatics Unit, Life Sciences Core Facilities
Street address P.O.B. 26
City Rehovot
ZIP/Postal code 76100
Country Israel
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (12)
GSM5082083 Control_Ribo-seq_1 [OligodT cleaned RNA]
GSM5082084 Control_Ribo-seq_2 [OligodT cleaned RNA]
GSM5082085 Control_Ti-seq_1 [OligodT cleaned RNA]
This SubSeries is part of SuperSeries:
GSE166744 eIF1-eIF4G1 inhibitors uncover alternative translation activation of stress-response genes via enhanced ribosome loading and 5’UTR translation
Relations
BioProject PRJNA701895
SRA SRP306426

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE166743_mars-seq_norm_counts.txt.gz 372.9 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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