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Status |
Public on Jul 07, 2022 |
Title |
eIF1-eIF4G1 inhibitors uncover alternative translation activation of stress-response genes via enhanced ribosome loading and 5’UTR translation [MARS-seq] |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
The Ribo-seq and TI-seq analysis following i14G1s (eI1-eIF4G1 inhibitors) treatments uncover opposing roles of eIF1-eIF4G1 and eIF4E-eIF4G1 in scanning-dependent and independent translation. Furthermore, i14G1s inhibition of eIF4G1-eIF1 resulted in translation activation of ER/UPR stress-response genes via enhanced ribosome loading, elevated 5’UTR translation at near cognate AUGs, and unexpected concomitant upregulation of coding-region translation.
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Overall design |
Ribo-seq and TI-seq analysis was performed either in HEK293T cells treated with either DMSO (vehicle control), i14G1-10 or i14G1-12.
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Contributor(s) |
Sehrawat U, Haimov O, Harush AT, Plotnikov A, Leshkowitz D, Dikstein R |
Citation missing |
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Submission date |
Feb 15, 2021 |
Last update date |
Jul 07, 2022 |
Contact name |
Dena Leshkowitz |
E-mail(s) |
dena.leshkowitz@weizmann.ac.il
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Organization name |
Weizmann Institute of Science
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Department |
Bioinformatics Unit, Life Sciences Core Facilities
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Street address |
P.O.B. 26
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City |
Rehovot |
ZIP/Postal code |
76100 |
Country |
Israel |
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Platforms (1) |
GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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Samples (12)
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GSM5082083 |
Control_Ribo-seq_1 [OligodT cleaned RNA] |
GSM5082084 |
Control_Ribo-seq_2 [OligodT cleaned RNA] |
GSM5082085 |
Control_Ti-seq_1 [OligodT cleaned RNA] |
GSM5082086 |
Control_Ti-seq_2 [OligodT cleaned RNA] |
GSM5082087 |
i14G1-10_Ribo-seq_1 [OligodT cleaned RNA] |
GSM5082088 |
i14G1-10_Ribo-seq_2 [OligodT cleaned RNA] |
GSM5082089 |
i14G1-10_Ti-seq_1 [OligodT cleaned RNA] |
GSM5082090 |
i14G1-10_Ti-seq_2 [OligodT cleaned RNA] |
GSM5082091 |
i14G1-12_Ribo-seq_1 [OligodT cleaned RNA] |
GSM5082092 |
i14G1-12_Ribo-seq_2 [OligodT cleaned RNA] |
GSM5082093 |
i14G1-12_Ti-seq_1 [OligodT cleaned RNA] |
GSM5082094 |
i14G1-12_Ti-seq_2 [OligodT cleaned RNA] |
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This SubSeries is part of SuperSeries: |
GSE166744 |
eIF1-eIF4G1 inhibitors uncover alternative translation activation of stress-response genes via enhanced ribosome loading and 5’UTR translation |
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Relations |
BioProject |
PRJNA701895 |
SRA |
SRP306426 |