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| Status |
Public on Aug 26, 2010 |
| Title |
The effect of ERG overexpression and ERG knockdown by shRNA in RWPE1 and VCAP cells, respectively |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The goal of this project was to analyze the global gene expression profiles of RWPE1 and VCAP cells following transfection of GFP, GFP-ERG at 48 and 72hrs time points and stable ERG shRNA, scramble shRNA, respectively.
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| Overall design |
RWPE1 cells were transfected with GFP or GFP-ERG. VCAP cells were transfected with ERG lenti-shRNA or scramble shRNA. Transfections were performed in duplicate. Total cellular RNA was isolated with Trizol and quality analysed by the bioanalyser kit.
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| Contributor(s) |
Gupta SK, Kristiina I, Henri S, Matthias N |
| Citation(s) |
20713528 |
| Submission date |
Jun 17, 2009 |
| Last update date |
Mar 20, 2017 |
| Contact name |
Santosh Kumar Gupta |
| E-mail(s) |
79santosh@gmail.com
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| Organization name |
VTT medical biotechnology
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| Department |
University of Turku
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| Lab |
VTT Medical Biotechnology (Olli Kallioniemi)
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| Street address |
4th floor pharma city
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| City |
Turku |
| ZIP/Postal code |
20520 |
| Country |
Finland |
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| Platforms (2) |
| GPL6255 |
Illumina humanRef-8 v2.0 expression beadchip |
| GPL6883 |
Illumina HumanRef-8 v3.0 expression beadchip |
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| Samples (12)
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| GSM416785 |
The effect of stable scramble shRNA in VCAP cells, array 1 |
| GSM416794 |
The effect of stable scramble shRNA in VCAP cells, array 2 |
| GSM416795 |
The effect of ERG knockdown by shRNA in VCAP cells, array 2 |
| GSM416796 |
The effect of ERG knockdown by shRNA in VCAP cells, array 1 |
| GSM417848 |
The effect of GFP overexpression in RWPE1 cells at 48hrs time point, array 1 |
| GSM417849 |
The effect of GFP overexpression in RWPE1 cells at 48hrs time point, array 2 |
| GSM417850 |
The effect of GFP overexpression in RWPE1 cells at 72hrs time point, array 1 |
| GSM417851 |
The effect of GFP overexpression in RWPE1 cells at 72hrs time point, array 2 |
| GSM417852 |
The effect of GFP-ERG overexpression in RWPE1 cells at 48hrs time point, array 1 |
| GSM417853 |
The effect of GFP-ERG overexpression in RWPE1 cells at 48hrs time point, array 2 |
| GSM417854 |
The effect of GFP-ERG overexpression in RWPE1 cells at 72hrs time point, array 1 |
| GSM417855 |
The effect of GFP-ERG overexpression in RWPE1 cells at 72hrs time point, array 2 |
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| Relations |
| BioProject |
PRJNA117385 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE16671_RAW.tar |
3.9 Mb |
(http)(custom) |
TAR |
| Processed data included within Sample table |
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