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Series GSE16656 Query DataSets for GSE16656
Status Public on Nov 19, 2009
Title Transcriptome analysis identifies molecular effectors of unconjugated bilirubin in human neuroblatoma SH-SY5Y cells: 24h
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The deposition of unconjugated bilirubin (UCB) in selected regions of the brain results in irreversible neuronal damage, or Bilirubin Encephalopathy (BE). Although UCB impairs a large number of cellular functions, the basic mechanisms of neurotoxicity have not yet been fully clarified. While cells can accumulate UCB by passive diffusion, cell protection may involve multiple mechanisms including the extrusion of the pigment as well as pro-survival homeostatic responses that are still unknown. The effects of UCB treatment to SH-SY5Y neuroblastoma cell line were examined by high density oligonucleotide microarrays. 230 genes were induced after 24 hours. A Gene Ontology (GO) analysis showed that a large group of UCB-induced genes were components of the ER stress response. Independent experimental validation of molecular events crucial for the ER stress response is presented. The results show that UCB exposure induces ER stress response as major intracellular homeostatic response in neuroblastoma cells in vitro. Our finding may provide valuable information for new therapeutic strategies in the treatment of BE.
Overall design We used high density oligonucleotide microarrays to analyze the gene expression profile of human neuroblastoma SH-SY5Y cells upon UCB treatment. Three replicates of UCB-treated cells were analyzed. As controls, we used three replicates of the same cells treated with DMSO only (the solvent used for UCB treatment).
Contributor(s) Roncaglia P
Citation(s) 19925663
Submission date Jun 17, 2009
Last update date Dec 06, 2018
Contact name Paola Roncaglia
Organization name SISSA/ISAS (International School for Advanced Studies)
Department Neurobiology
Street address via Bonomea 265
City Trieste
State/province TS
ZIP/Postal code 34136
Country Italy
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (6)
GSM417814 SH-SY5Ycells_bilirubin_treated_140nM_24hours_rep1
GSM417815 SH-SY5Ycells_bilirubin_treated_140nM_24hours_rep2
GSM417816 SH-SY5Ycells_bilirubin_treated_140nM_24hours_rep3
This SubSeries is part of SuperSeries:
GSE16768 Transcriptome analysis identifies molecular effectors of unconjugated bilirubin in human neuroblastoma SH-SY5Y cells
BioProject PRJNA122787

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Supplementary file Size Download File type/resource
GSE16656_RAW.tar 12.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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