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Series GSE166176 Query DataSets for GSE166176
Status Public on Feb 05, 2021
Title Bulk and single RNA sequencing on the role of Igf2bp2 in myeloid-biased HSC
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Using RNA sequencing analysis on myeloid biased HSC, we compared gene expression profiles between WT and Igf2bp2 knock-out mice at young and old age. 1421 differentially expressed genes were identified in young myeloid biased HSC from Igf2bp2 knock-out mice compared to young WT; however, only 26 differentially expressed genes were identified in old myeloid biased HSC from Igf2bp2 knock-out mice compared to old WT. Compared to young WT myeloid biased HSC, myeloid biased HSC from young Igf2bp2 knock-out exhibits reduced expression of genes involved in metabolism and translation. In addition, single cell RNA sequencing analysis of myeloid biased HSC in young wildtype mice identified nine sub-clusters. HSCs from a sub-cluster with high expression of Igf2bp2 exhibit up-regulated IGF/PI3K/AKT signaling and increased expression of genes involved in HSC quiescence and maintenance. Altogether, the study provides a correlation and mechanism to understand the role of Igf2bp2 in HSC function and aging.
 
Overall design RNA sequencing of myeloid-biased HSC from Wild Type and Igf2bp2-/- mice at young and old; single cell RNA sequencing of myeloid-biased HSC from Wild Type at 6 week old
 
Contributor(s) Suo M, Bens M, Görlich I
Citation(s) 35231105
Submission date Feb 04, 2021
Last update date May 01, 2024
Contact name Marthe Behrends
Organization name Leibniz Institute on Aging – Fritz Lipmann Institute
Lab Rudolph
Street address Beutenbergstraße 11
City Jena
ZIP/Postal code 07745
Country Germany
 
Platforms (2)
GPL19057 Illumina NextSeq 500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (17)
GSM5065181 O_KO_480
GSM5065182 O_KO_481
GSM5065183 O_KO_564
Relations
BioProject PRJNA699541
SRA SRP304738

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE166176_RAW.tar 280.2 Mb (http)(custom) TAR (of TAR)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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