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| Status |
Public on Feb 03, 2021 |
| Title |
Mettl3/m6A promotes translation of pathogenic mRNAs to mediate cystogenesis in mouse models of ADPKD |
| Organism |
Mus musculus |
| Experiment type |
Other
|
| Summary |
Purpose: Determine the differential m6A methylation pattern of Mettl3 between wildtype, Pkd1F/RC-KO and Pkd1F/RC-Mettl3-DKO mouse kidneys. Methods: m6A RNA IP using the Magna MeRIP™ m6A Kit (EMD Millipore, catalog #17-10499) was performed on P18 wildtype, Pkd1F/RC-KO and Pkd1F/RC-Mettl3-DKO mouse kidney samples. Two biological replicate samples from each genotype were sequenced. Each sample was a pool of 6 kidneys of the same genotype. The input samples were also sequenced to obtain mRNA profiles. Results: 133 mRNAs were found to be differentially hypermethylated in the Pkd1-KO kidneys compared to the control kidneys and differentially hypomethylated in the Pkd1-Mettl3-DKO kidneys compared to the Pkd1-KO kidneys. Two key pathogenic mRNAs namely, c-Myc and Avpr2 were identified and validated as Mettl3 targets. The mRNA transcript levels were unchanged between Pkd1-KO and Pkd1-Mettl3-DKO kidneys. Conclusion: Mettl3/m6A promotes translation of pathogenic mRNAs to mediate cystogenesis in mouse models of ADPKD.
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| Overall design |
m6A methylation profile of P18 wildtype, Pkd1F/RC-KO and Pkd1F/RC-Mettl3-KO murine kidneys
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| Contributor(s) |
Patel V, Ramalingam H |
| Citation missing |
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| |
| Submission date |
Feb 01, 2021 |
| Last update date |
Feb 13, 2021 |
| Contact name |
harini ramalingam |
| E-mail(s) |
harini.ramalingam@utsouthwestern.edu
|
| Phone |
9727301211
|
| Organization name |
UTSW
|
| Department |
IMN
|
| Lab |
F5.206
|
| Street address |
5323 Harry Hines Boulevard
|
| City |
Dallas |
| State/province |
Texas |
| ZIP/Postal code |
75390 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA698647 |
| SRA |
SRP304189 |
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