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Series GSE165489 Query DataSets for GSE165489
Status Public on Dec 31, 2024
Title Blood gene expression changes in response to exposure to dust containing silica
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Occupational exposure to dust containing crystalline silica may result in serious adverse health effects including silicosis and cancer. Previous studies which employed animal models for inhalation exposure to crystalline silica revealed changes in blood gene expression profiles in association with the silica-induced lung toxicity. Currently, global gene expression profiles were determined in the whole blood samples obtained from control (not exposed to dust) and dust containing silica exposed individuals with or without clinically identified silicosis. Differences in the blood gene expression profiles were detected in the blood samples obtained from the control and the silica-containing dust exposed individuals. Between the two groups of the silica-containing dust exposed individuals, the number of significantly differentially expressed genes was more in the blood samples obtained from those with silicosis.
 
Overall design Whole blood samples collected from individuals who were exposed to dust containing silica were used in the study. Blood samples collected from an unexposed, matching cohort were used as the controls (CTl2 to CTl60) (n = 23). The lung function and silicosis status of the study participants were determined by spirometry and chest x-ray, respectively. Based on the results of chest x-ray, the silica exposed subjects were classified into two groups – dust exposed with silicosis (TESC_02 to TESC_40) (n = 30) and dust exposed without silicosis (TESC_42 to TESC_89) (n = 33). Gene expression profiles were determined by next generation sequence analysis in the blood samples collected from the subjects.
 
Contributor(s) Joseph P, Lowe E
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jan 25, 2021
Last update date Dec 31, 2024
Contact name Pius Joseph
E-mail(s) pcj5@cdc.gov
Phone 3042856240
Organization name National Institute for Occupational Safety and Health (NIOSh)
Department Health Effects Laboratory Division/Toxicology and Molecular Biology Branch
Lab Molecular Carcinogenesis Laboratory
Street address 1095 Willowdale Road
City Morgantown
State/province WV
ZIP/Postal code 26505
Country USA
 
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (86)
GSM5034544 TESC_02
GSM5034545 TESC_03
GSM5034546 TESC_04
Relations
BioProject PRJNA694679
SRA SRP303197

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE165489_RAW.tar 1.6 Gb (http)(custom) TAR (of TAR)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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