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Series GSE165042 Query DataSets for GSE165042
Status Public on Jul 23, 2021
Title CLOCKWORK ORANGE promotes CLOCK-CYCLE activation via the Drosophila ortholog of CLOCK INTERACING PROTEIN, CIRCADIAN (ChIP-Seq)
Organism Drosophila melanogaster
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary CWO reinforces PER-TIM repression of core clock gene transcription by antagonizing CLK-CYC binding to E-boxes, but also functions to promote CLK-CYC transcription of core clock genes. To determine the relationship between CWO and CLK-CYC binding across the genome, we identified CWO and CLK binding sites via ChIP-seq.
 
Overall design ChIP-seq: to identify CWO target genes, HA antibody was used to IP CWO-HA from heads of cwo-HA; cwo5073 flies collected during transcriptional repression at Zeitgeber Time 2 (ZT2, where ZT0 is lights-on and ZT12 is lights-off during an LD cycle) and transcription activation at ZT14 for ChIP-seq analysis. CLK was IPed with anti-CLK antibody in parallel
 
Contributor(s) Rivas GS, Zhou J, Merlin C, Hardin PE
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Submission date Jan 18, 2021
Last update date Jul 26, 2021
Contact name Paul Hardin
E-mail(s) grivas@bio.tamu.edu
Phone 4075419203
Organization name Texas A&M University
Street address 3258 TAMU Dept of Biology Buttler Hall room 100H
City College Station
State/province Texas
ZIP/Postal code 77843
Country USA
 
Platforms (1)
GPL17275 Illumina HiSeq 2500 (Drosophila melanogaster)
Samples (6)
GSM5025010 ZT2_INPUT
GSM5025011 ZT14_INPUT
GSM5025012 ZT2_HA
This SubSeries is part of SuperSeries:
GSE165044 CLOCKWORK ORANGE promotes CLOCK-CYCLE activation via the putative Drosophila ortholog of CLOCK INTERACTING PROTEIN CIRCADIAN
Relations
BioProject PRJNA693023
SRA SRP302238

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE165042_RAW.tar 704.0 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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