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| Status |
Public on Jul 23, 2021 |
| Title |
CLOCKWORK ORANGE promotes CLOCK-CYCLE activation via the Drosophila ortholog of CLOCK INTERACING PROTEIN, CIRCADIAN (ChIP-Seq) |
| Organism |
Drosophila melanogaster |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
CWO reinforces PER-TIM repression of core clock gene transcription by antagonizing CLK-CYC binding to E-boxes, but also functions to promote CLK-CYC transcription of core clock genes. To determine the relationship between CWO and CLK-CYC binding across the genome, we identified CWO and CLK binding sites via ChIP-seq.
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| Overall design |
ChIP-seq: to identify CWO target genes, HA antibody was used to IP CWO-HA from heads of cwo-HA; cwo5073 flies collected during transcriptional repression at Zeitgeber Time 2 (ZT2, where ZT0 is lights-on and ZT12 is lights-off during an LD cycle) and transcription activation at ZT14 for ChIP-seq analysis. CLK was IPed with anti-CLK antibody in parallel
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| Contributor(s) |
Rivas GS, Zhou J, Merlin C, Hardin PE |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jan 18, 2021 |
| Last update date |
Jul 26, 2021 |
| Contact name |
Paul Hardin |
| E-mail(s) |
grivas@bio.tamu.edu
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| Phone |
4075419203
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| Organization name |
Texas A&M University
|
| Street address |
3258 TAMU Dept of Biology Buttler Hall room 100H
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| City |
College Station |
| State/province |
Texas |
| ZIP/Postal code |
77843 |
| Country |
USA |
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| Platforms (1) |
| GPL17275 |
Illumina HiSeq 2500 (Drosophila melanogaster) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE165044 |
CLOCKWORK ORANGE promotes CLOCK-CYCLE activation via the putative Drosophila ortholog of CLOCK INTERACTING PROTEIN CIRCADIAN |
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| Relations |
| BioProject |
PRJNA693023 |
| SRA |
SRP302238 |
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