|
| Status |
Public on Aug 10, 2021 |
| Title |
Targeted transposon screening in bacteria by CRISPR/Cas12k-guided transposase |
| Organism |
Pseudomonas aeruginosa PAO1 |
| Experiment type |
Other
|
| Summary |
Here, we report a CRISPR/Cas12k-transposon-assisted genome engineering (CTAGE) method that allows for high-throughput site-specific mutagenesis in microbial genomes. Exploiting the powerful CTAGE technique, we construct a site-specific transposon mutant library focusing on all the possible transcription factors (TFs) in Pseudomonas aeruginosa, enabling comprehensive identification of essential genes and new factors for antibiotic resistance.
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| |
|
| Overall design |
Examination resistance gene of imipenem, ceftazidime and ofloxacin in Pseudomonas aeruginosa.
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| |
|
| Contributor(s) |
Weizhong C, Zehui R |
| Citation(s) |
34469724 |
| |
| Submission date |
Jan 13, 2021 |
| Last update date |
Nov 09, 2021 |
| Contact name |
ren zehui |
| E-mail(s) |
renzh6@mail2.sysu.edu.cn
|
| Organization name |
SUN YAT-SEN UNIVERSITY
|
| Street address |
No. 135, Xingang Xi Road
|
| City |
Guangzhou |
| ZIP/Postal code |
510275 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL28604 |
HiSeq X Ten (Pseudomonas aeruginosa PAO1) |
|
| Samples (10)
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|
| Relations |
| BioProject |
PRJNA691969 |
| SRA |
SRP301647 |
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