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Series GSE164476 Query DataSets for GSE164476
Status Public on Aug 24, 2021
Title Loss of macrophage miR-146b with aging contributes to inflammation and mitochondrial dysfunction
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary Macrophages undergo programmatic changes with age leading to altered cytokine polarization and immune dysfunction, shifting these critical immune cells from protective sentinels to disease promoters. The molecular mechanisms underlying macrophage inflammaging are poorly understood. Using an unbiased RNA-seq approach, we identified miR-146b as the only microRNA whose expression progressively and unidirectionally declined with age in murine macrophages. miR-146b deficiency led to altered macrophage cytokine expression and reduced mitochondrial metabolic activity, two hallmarks of cellular aging. Single-cell RNA sequencing identified patterns of altered inflammation and interferon gamma signaling in miR-146b deficient macrophages. Identification of miR-146b as a potential regulator of macrophage aging provides novel insights into immune dysfunction associated with aging.
Overall design Peritoneal macrophages were harvested from 3-, 6-, 12-, 18-, 24- and 30-month-old C57Bl/6 mice or Lyz2; miR-146b macrophage/monocyte conditional knockout and littermate control mice.
Contributor(s) Apte RS, Santeford A, Sergushichev A, Ruzycki PA
Citation(s) 34423778
Submission date Jan 08, 2021
Last update date Dec 05, 2022
Contact name Andrea Santeford
Phone 314-362-6690
Organization name Washington University School of Medicine
Department Opthalmology
Lab Rajendra S. Apte
Street address 660 S. Euclid Ave.
City Saint Louis
State/province MO
ZIP/Postal code 63021
Country USA
Platforms (3)
GPL14602 AB SOLiD 4 System (Mus musculus)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (18)
GSM5011751 3mo
GSM5011752 6mo
GSM5011753 12mo
BioProject PRJNA690809
SRA SRP300927

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE164476_RAW.tar 247.7 Mb (http)(custom) TAR (of TAR)
GSE164476_counts.txt.gz 243.3 Kb (ftp)(http) TXT
GSE164476_mirSeq_3-30months.txt.gz 24.9 Kb (ftp)(http) TXT
GSE164476_mm_ref_new.fa.gz 735.8 Mb (ftp)(http) FA
GSE164476_mm_ref_new_2.fa.gz 736.2 Mb (ftp)(http) FA
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file

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