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| Status |
Public on Dec 27, 2020 |
| Title |
Alpha-tocopherol exerts protective function against the mucotoxicity of particulate matter in amphibian and human goblet cells |
| Organisms |
Bombina orientalis; Xenopus laevis |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Exposure to particulate matter (PM) in ambient air is known to increase the risk of cardiovascular disorders and mortality. The cytotoxicity of PM is mainly due to the abnormal increase of reactive oxygen species (ROS), which damage cellular components such as DNA, RNA, and proteins. The correlation between PM exposure and human disorders, including mortality, is based on long-term exposure. In this study we have investigated acute responses of mucus-secreting goblet cells upon exposure to PM derived from a heavy diesel engine. To this end, we employed the mucociliary epithelium of amphibian embryos and human Calu-3 cells to examine PM mucotoxicity. Our data suggest that acute exposure to PM significantly impairs mucus secretion and results in the accumulation of mucus vesicles in the cytoplasm of goblet cells. RNA-seq analysis revealed that acute responses to PM exposure significantly altered gene expression patterns; however, known regulators of mucus production and the secretory pathway were not significantly altered. Interestingly, pretreatment with α-tocopherol nearly recovered the hyposecretion of mucus from both amphibian and human goblet cells. We believe this study demonstrates the mucotoxicity of PM and the protective function of α-tocopherol on mucotoxicity caused by acute PM exposure from heavy diesel engines.
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| Overall design |
For each species (X. laevis and B. orientalis), we treated 100 ug/mL of the particulate matters (PM) to whole embryo. For each condition, we used duplicated biological replicates. To evaluate the transcriptonal response among time, we sampled at two time points: 3 hours after PM treatment, and 9 hours after the treatment.
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| Contributor(s) |
Kwon T, Yang H, Sim HJ, Cho H, Bang WY, Kim HE, Kwon TK, Park TJ |
| Citation(s) |
32277121 |
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| Submission date |
Dec 26, 2020 |
| Last update date |
Oct 17, 2023 |
| Contact name |
Taejoon Kwon |
| E-mail(s) |
tkwon@unist.ac.kr
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| Organization name |
Ulsan National Institute of Science and Technology
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| Department |
Department of Biomedical Engineering
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| Street address |
50 Unist-gil
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| City |
Ulsan |
| ZIP/Postal code |
44919 |
| Country |
South Korea |
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| Platforms (2) |
| GPL18936 |
Illumina HiSeq 2500 (Xenopus laevis) |
| GPL26992 |
Illumina HiSeq 2500 (Bombina orientalis) |
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| Samples (16)
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| GSM4988988 |
B. orientalis embryo, 3hr, control, rep 1 |
| GSM4988989 |
B. orientalis embryo, 3hr, control, rep 2 |
| GSM4988990 |
B. orientalis embryo, 9hr, control, rep 1 |
| GSM4988991 |
B. orientalis embryo, 9hr, control, rep 2 |
| GSM4988992 |
B. orientalis embryo, 3hr, 100 ug/mL heavy diesel particulate matter treatment, rep 1 |
| GSM4988993 |
B. orientalis embryo, 3hr, 100 ug/mL heavy diesel particulate matter treatment, rep 2 |
| GSM4988994 |
B. orientalis embryo, 9hr, 100 ug/mL heavy diesel particulate matter treatment, rep 1 |
| GSM4988995 |
B. orientalis embryo, 9hr, 100 ug/mL heavy diesel particulate matter treatment, rep 2 |
| GSM4988996 |
X. laevis embryo, 3hr, control, rep 1 |
| GSM4988997 |
X. laevis embryo, 3hr, control, rep 2 |
| GSM4988998 |
X. laevis embryo, 9hr, control, rep 1 |
| GSM4988999 |
X. laevis embryo, 9hr, control, rep 2 |
| GSM4989000 |
X. laevis embryo, 3hr, 100 ug/mL heavy diesel particulate matter treatment, rep 1 |
| GSM4989001 |
X. laevis embryo, 3hr, 100 ug/mL heavy diesel particulate matter treatment, rep 2 |
| GSM4989002 |
X. laevis embryo, 9hr, 100 ug/mL heavy diesel particulate matter treatment, rep 1 |
| GSM4989003 |
X. laevis embryo, 9hr, 100 ug/mL heavy diesel particulate matter treatment, rep 2 |
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| Relations |
| BioProject |
PRJNA687987 |
| SRA |
SRP299616 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE163875_BOMOR+XENLA+PM.kallisto.zip.gz |
11.1 Mb |
(ftp)(http) |
ZIP |
| GSE163875_Bombina_orientalis.a2020_08.cdna.fa.gz |
7.9 Mb |
(ftp)(http) |
FA |
| GSE163875_XENLA_XB201901_tx_annot.fa.gz |
44.1 Mb |
(ftp)(http) |
FA |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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