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Status |
Public on Nov 03, 2021 |
Title |
Integrative proteome analyses implicate aberrant RNA splicing impairs developmental potential of aged mouse oocytes |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Aging has many effects on female reproductive system, among which declined oocyte quality and impaired embryo developmental potential are the most important factors affecting female fertility. However, the mechanisms of oocyte aging have not yet been fully understood due to the complex and multifactorial nature of this physiological process. Here, we selected normal reproductively aging female mice as research object and constructed protein expression profile of metaphase II (MII) oocytes from three age groups. A total of 187 differentially expressed (DE) proteins were identified, and bioinformatics analysis showed that these DE proteins were highly enriched in the RNA splicing. Next, RNA-seq was performed on 2-cell embryos from these three age groups, splicing analysis showed that there were a total of 1375 differentially spliced genes (DSGs) and 2363 differentially spliced events (DSEs). DSGs in the reproductive aging groups versus the younger group were enriched in biological processes related to DNA damage repair/response. Binding motif analysis suggested PUF60 may be the core splicing factor that causes the decline of the developmental potential of oocytes in reproductive aging mice, and changing the splicing pattern of its potential downstream DSG Cdk9 could partially mimic phenotypes in the reproductive aging groups. Taken together, our study suggested the abnormal expression of splicing regulation protein in aging MII oocytes will affect the splicing of nascent RNA after zygotic genome activation in 2-cell embryos, leading to the production of abnormally spliced transcripts of some key genes associated with DNA damage repair/response, thus affected the developmental potential of aged oocytes.
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Overall design |
Total 14 samples from 8-10w, 6-8m and 10-12m mice from MII oocytes and 2-cell embryos.
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Contributor(s) |
Li M |
Citation(s) |
34582091 |
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Submission date |
Dec 22, 2020 |
Last update date |
Nov 04, 2021 |
Contact name |
Wenjie Shu |
E-mail(s) |
shuwj@bmi.ac.cn
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Organization name |
Beijing Institute of Radiation Medicine
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Street address |
Taiping Road 27
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City |
Beijing |
ZIP/Postal code |
100850 |
Country |
China |
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Platforms (1) |
GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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Samples (15)
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Relations |
BioProject |
PRJNA687264 |
SRA |
SRP298968 |