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| Status |
Public on Dec 01, 2021 |
| Title |
Genome-wide distribution of Tus-GFP in KRX E. coli |
| Organism |
Escherichia coli KRX |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We examined the chromosomal distribution of GFP-tagged Tus (Tus-GFP) in exponentially growing E. coli (KRX) using chromosome immunoprecipitation (ChIP)-Seq. Our ChIP-seq allowed delineation of a refined minimal replication fork trap within E. coli comprising two clusters of three Ter sites
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| |
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| Overall design |
Three biological replicates of immunoprecipitated DNA (ChIP) are shown each consisting of 2-3 technical replicates. Input DNA (Pre-Immunoprecipitated sample) was subjected to the same protocol and used as a control
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| Contributor(s) |
Schaeffer P, Moreau M, Toft C |
| Citation(s) |
34948327 |
| |
| Submission date |
Dec 21, 2020 |
| Last update date |
Jan 10, 2022 |
| Contact name |
Patrick Schaeffer |
| E-mail(s) |
patrick.schaeffer@jcu.edu.au
|
| Organization name |
James Cook University
|
| Department |
CPHMVS
|
| Lab |
Schaeffer
|
| Street address |
1 James Cook Drive
|
| City |
Douglas |
| State/province |
QLD |
| ZIP/Postal code |
4814 |
| Country |
Australia |
| |
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| Platforms (1) |
| GPL29521 |
Illumina MiSeq (Escherichia coli KRX) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA687054 |
| SRA |
SRP298810 |
