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| Status |
Public on Oct 01, 2022 |
| Title |
TENT5 cytoplasmic non-canonical poly(A) polymerases regulate the innate immune response in animals |
| Organisms |
Caenorhabditis elegans; Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Innate immunity, the first line of host defense against pathogens, is tightly regulated both transcriptionally and post-transcriptionally. Through global transcriptome and proteome analyses in Caenorhabditis elegans, we uncover a modulation of the expression of secreted innate immunity effector proteins by TENT5, one of a recently described family of cytoplasmic poly(A) polymerases. Direct RNA sequencing revealed that TENT-5 polyadenylates mRNAs with signal peptide-encoding sequences, that are translated at the endoplasmic reticulum. Loss of tent-5 function makes worms more susceptible to bacterial infection. Importantly, we demonstrate that the function of TENT-5 in innate immunity is evolutionarily conserved, as its orthologs, TENT5A and TENT5C are induced during macrophage activation and polyadenylate mRNAs, some of which are of genes orthologous to C. elegans TENT-5 targets. In summary, our study reveals cytoplasmic polyadenylation to be a previously unknown component of the post-transcriptional regulation of innate immunity in animals.
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| Overall design |
Global transcriptomic analysis of L4-stage tent-5-deficient (TENT-5) and wild-type (WT) worms grown on non-pathogenic Escherichia coli strain HB101 (HB) or infected with Staphylococcus aureus NCTC 8325 (SA) for 8 hours. Other tent-5 IDs: F55A12.9, pqn-44. Transcriptomic analysis of bone marrow-derived macrophages (BMDM) treated with LPS for 8h compared to untreated samples.
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| Contributor(s) |
Liudkovska V, Krawczyk PS, Mroczek S, Dziembowski A |
| Citation(s) |
36383655 |
| |
| Submission date |
Dec 19, 2020 |
| Last update date |
Jan 02, 2023 |
| Contact name |
Andrzej Dziembowski |
| E-mail(s) |
adziembowski@iimcb.gov.pl
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| Organization name |
International Institute Of Molecular And Cell Biology In Warsaw
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| Lab |
Laboratory of RNA Biology
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| Street address |
4 Ks. Trojdena Street
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| City |
Warsaw |
| ZIP/Postal code |
02-109 |
| Country |
Poland |
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| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL19757 |
Illumina NextSeq 500 (Caenorhabditis elegans) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA686670 |
| SRA |
SRP298574 |
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