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Series GSE163549 Query DataSets for GSE163549
Status Public on Oct 01, 2022
Title TENT5 cytoplasmic non-canonical poly(A) polymerases regulate the innate immune response in animals
Organisms Caenorhabditis elegans; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Innate immunity, the first line of host defense against pathogens, is tightly regulated both transcriptionally and post-transcriptionally. Through global transcriptome and proteome analyses in Caenorhabditis elegans, we uncover a modulation of the expression of secreted innate immunity effector proteins by TENT5, one of a recently described family of cytoplasmic poly(A) polymerases. Direct RNA sequencing revealed that TENT-5 polyadenylates mRNAs with signal peptide-encoding sequences, that are translated at the endoplasmic reticulum. Loss of tent-5 function makes worms more susceptible to bacterial infection. Importantly, we demonstrate that the function of TENT-5 in innate immunity is evolutionarily conserved, as its orthologs, TENT5A and TENT5C are induced during macrophage activation and polyadenylate mRNAs, some of which are of genes orthologous to C. elegans TENT-5 targets. In summary, our study reveals cytoplasmic polyadenylation to be a previously unknown component of the post-transcriptional regulation of innate immunity in animals. 
Overall design Global transcriptomic analysis of L4-stage tent-5-deficient (TENT-5) and wild-type (WT) worms grown on non-pathogenic Escherichia coli strain HB101 (HB) or infected with Staphylococcus aureus NCTC 8325 (SA) for 8 hours. Other tent-5 IDs: F55A12.9, pqn-44. Transcriptomic analysis of bone marrow-derived macrophages (BMDM) treated with LPS for 8h compared to untreated samples.
Contributor(s) Liudkovska V, Krawczyk PS, Mroczek S, Dziembowski A
Citation(s) 36383655
Submission date Dec 19, 2020
Last update date Jan 02, 2023
Contact name Paweł S Krawczyk
Organization name International Institute Of Molecular And Cell Biology In Warsaw
Lab Laboratory of RNA Biology
Street address 4 Ks. Trojdena Street
City Warsaw
ZIP/Postal code 02-109
Country Poland
Platforms (2)
GPL19057 Illumina NextSeq 500 (Mus musculus)
GPL19757 Illumina NextSeq 500 (Caenorhabditis elegans)
Samples (18)
GSM4981575 HB_WT_1
GSM4981576 HB_WT_2
GSM4981577 HB_WT_3
BioProject PRJNA686670
SRA SRP298574

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Supplementary file Size Download File type/resource
GSE163549_BMDM_LPS_DESeq2_normalized_counts.tsv.gz 391.5 Kb (ftp)(http) TSV
GSE163549_C_elegans_E_coli_DESeq2_normalized_counts.tsv.gz 216.9 Kb (ftp)(http) TSV
GSE163549_C_elegans_S_aureus_DESeq2_normalized_counts.tsv.gz 221.7 Kb (ftp)(http) TSV
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