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Series GSE163249 Query DataSets for GSE163249
Status Public on Dec 02, 2021
Title Targeted inhibition of LIFR enhances therapeutic efficacy of HDAC inhibitors in triple negative breast cancer
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Here, we examined the therapeutic utility of EC359 in improving the therapeutic efficacy of HDACi in TNBC models. BT-549 cells were treated with vehicle (DMSO), EC359, HDACi(Vorinostat), EC359+HDACi and the RNA was isolated and utilized for RNA-seq analysis. Our results demonstrated that the beneficial effect of the EC359+HDACi involves regulation of multiple genes that involved in several pathways including apoptosis, metabolism and cell cycle.
Overall design Total RNA was isolated from BT-549 cells. Illumina TruSeq RNA Sample Preparation was performed following manufacturer's protocol. Samples were run on an Illumina HiSeq 3000 in duplicate. The combined raw reads were aligned to UCSC hg19 by Tophat. Genes were annotated and quantified by HTSeq-DESeq pipeline.
Contributor(s) Viswanadhapalli S, Chen Y, Zou Y, Vadlamudi RK
Citation(s) 34716410
Submission date Dec 15, 2020
Last update date Dec 03, 2021
Contact name YI ZOU
Organization name UTHSA-GCCRI
Street address 8403 Floyd Curl Drive
State/province TX
ZIP/Postal code 78229
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (8)
GSM4975597 BT_549_Vehicle_1
GSM4975599 BT_549_Vehicle_2
GSM4975601 BT_549_EC359+SAHA_1
BioProject PRJNA685420
SRA SRP298026

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE163249_RAW.tar 760.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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