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Status |
Public on Jan 07, 2022 |
Title |
Single cell RNA sequencing data of Side Population and Non-Side Population tumor cells from a mouse model of undifferentiated pleomorphic sarcoma |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
We report the application of single-cell RNA sequencing data on Side Population (SP) cells and their Non-Side Population (NSP) counterparts in a mouse model of undifferentiated pleomorphic sarcoma (UPS). SP cells exhibit tumor propagting cell properties characterized by enhanced tumorigenicity and self-renewal potential. The purpose of this experiment is to investigate cellular heterogeneity at the gene expression level in UPS tumors and lineage relationships between different subpopulation of tumor cells. We generated the gene expression profiles of individual cells in the SP and NSP compartments of mouse UPS.
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Overall design |
Mouse UPS tumors are sorted into SP and NSP populations using flow cytometery and the single-cell RNA sequencing is performed for the sorted populations
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Contributor(s) |
Tang YJ, Xiang Y, Diao Y, Alman B |
Citation(s) |
34618689 |
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Submission date |
Dec 08, 2020 |
Last update date |
Jan 07, 2022 |
Contact name |
Benjamin Alman |
E-mail(s) |
ben.alman@duke.edu
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Organization name |
Duke university
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Street address |
Levine Science Research Center,308 Research Drive
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City |
Durham |
State/province |
NC |
ZIP/Postal code |
27710 |
Country |
USA |
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Platforms (1) |
GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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Samples (6)
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Relations |
BioProject |
PRJNA683583 |
SRA |
SRP297063 |