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Series GSE162847 Query DataSets for GSE162847
Status Public on Jan 07, 2022
Title Single cell RNA sequencing data of Side Population and Non-Side Population tumor cells from a mouse model of undifferentiated pleomorphic sarcoma
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We report the application of single-cell RNA sequencing data on Side Population (SP) cells and their Non-Side Population (NSP) counterparts in a mouse model of undifferentiated pleomorphic sarcoma (UPS). SP cells exhibit tumor propagting cell properties characterized by enhanced tumorigenicity and self-renewal potential. The purpose of this experiment is to investigate cellular heterogeneity at the gene expression level in UPS tumors and lineage relationships between different subpopulation of tumor cells. We generated the gene expression profiles of individual cells in the SP and NSP compartments of mouse UPS.
 
Overall design Mouse UPS tumors are sorted into SP and NSP populations using flow cytometery and the single-cell RNA sequencing is performed for the sorted populations
 
Contributor(s) Tang YJ, Xiang Y, Diao Y, Alman B
Citation(s) 34618689
Submission date Dec 08, 2020
Last update date Jan 07, 2022
Contact name Benjamin Alman
E-mail(s) ben.alman@duke.edu
Organization name Duke university
Street address Levine Science Research Center,308 Research Drive
City Durham
State/province NC
ZIP/Postal code 27710
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (6)
GSM4962845 SP
GSM4962846 NSP
GSM4962847 840-NSP
Relations
BioProject PRJNA683583
SRA SRP297063

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE162847_cellranger_gene_count_table_mm10.tar.gz 335.9 Mb (ftp)(http) TAR
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Processed data are available on Series record

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