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Series GSE162845 Query DataSets for GSE162845
Status Public on Jun 01, 2022
Title Next generation sequencing of human cholangiocarcinoma cells and associated stem-like component
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The goal of this study is to provide a global transcriptome profiling (RNA-seq) of stem-like subset in human cholangiocarcinoma (CCA). Functional enrichment of CCA stem-like subset was performed by 3D sphere culture (SPH) in CCA cell lines. Comparison to parental CCA cells grown as 2D monolayer is provided.
Overall design mRNA profile of 3D cholangio-spheres (SPH) compared to parental 2D monolayer (MON) cells of 4 different cholangiocarcinoma cell lines
Contributor(s) Ramazzotti M, Marra F, Raggi C, Peano C, Correnti M
Citation(s) 33484774
Submission date Dec 08, 2020
Last update date Jun 01, 2022
Contact name Matteo Ramazzotti
Organization name University of Florence
Department Experimental and Clinical Biomedical Sciences
Street address viale Morgagni 50
City Firenze
ZIP/Postal code 50134
Country Italy
Platforms (2)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL21697 NextSeq 550 (Homo sapiens)
Samples (26)
GSM4962795 MTCHC01_spheroid_1
GSM4962796 HUCCT1_spheroid_1
GSM4962797 CCLP1_spheroid_1
BioProject PRJNA683582
SRA SRP297050

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Supplementary file Size Download File type/resource
GSE162845_SalmonData.rData 97.8 Mb (ftp)(http) RDATA
GSE162845_gene_abundance.txt.gz 4.2 Mb (ftp)(http) TXT
GSE162845_transcript_abundance.txt.gz 13.9 Mb (ftp)(http) TXT
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Processed data are available on Series record

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