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Status |
Public on Jun 01, 2022 |
Title |
Next generation sequencing of human cholangiocarcinoma cells and associated stem-like component |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
The goal of this study is to provide a global transcriptome profiling (RNA-seq) of stem-like subset in human cholangiocarcinoma (CCA). Functional enrichment of CCA stem-like subset was performed by 3D sphere culture (SPH) in CCA cell lines. Comparison to parental CCA cells grown as 2D monolayer is provided.
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Overall design |
mRNA profile of 3D cholangio-spheres (SPH) compared to parental 2D monolayer (MON) cells of 4 different cholangiocarcinoma cell lines
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Contributor(s) |
Ramazzotti M, Marra F, Raggi C, Peano C, Correnti M |
Citation(s) |
33484774 |
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Submission date |
Dec 08, 2020 |
Last update date |
Jun 01, 2022 |
Contact name |
Matteo Ramazzotti |
E-mail(s) |
matteo.ramazzotti@unifi.it
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Organization name |
University of Florence
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Department |
Experimental and Clinical Biomedical Sciences
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Street address |
viale Morgagni 50
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City |
Firenze |
ZIP/Postal code |
50134 |
Country |
Italy |
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Platforms (2) |
GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
GPL21697 |
NextSeq 550 (Homo sapiens) |
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Samples (26)
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Relations |
BioProject |
PRJNA683582 |
SRA |
SRP297050 |