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Series GSE162832 Query DataSets for GSE162832
Status Public on Jan 12, 2021
Title PDA Indolylmaleimides Induce Anti-Tumor Effects in Prostate Carcinoma Cell Lines through Mitotic Death
Organisms Homo sapiens; Canis lupus familiaris
Experiment type Expression profiling by high throughput sequencing
Summary Introduction: Castrate resistant prostate cancer in men shares several characteristics with late-stage canine prostate cancer. Consequently, dogs became a model organism for the study of the human disease. Due to current insufficient therapies, evaluation of novel therapeutic agents for late-stage prostate cancer is of considerable interest for both species.
Methods: In this interdisciplinary study we characterized the influence of two indolylmaleimides (PDA-66 and PDA-377) on human and canine prostate carcinoma cell lines. For this purpose, we analyzed morphology, proliferation and apoptosis, recorded live cell imaging movies and performed whole transcriptome sequencing.
Results: While PDA-377 showed only weak growth inhibition on the human prostate cancer cell lines, PDA-66 inhibited proliferation and induced apoptosis in all prostate carcinoma cell lines with concentrations in the low micromolar range. Morphological characterization and whole transcriptome sequencing revealed that PDA-66 induces mitotic death through its microtubule-depolymerizing ability.
Discussion: PDA-66 appears to be a worthwhile anti-mitotic agent for further evaluation. The similarities in cellular and molecular response observed in the cell lines of both origins form a solid basis for the use of canine PCa in vivo models to provide valuable transferable findings to the benefit of both species.
Overall design Examination of the mediated effects by PDA-66 application on human and canine prostate carcinoma cell lines
Contributor(s) Schille JT, Nolte I, Beck J, Jilani D, Roolf C, Pews-Davtyan A, Rolfs A, Henze L, Beller M, Brenig B, Junghanss C, Schütz E, Murua Escobar H
Citation(s) 33553272
Submission date Dec 08, 2020
Last update date Apr 13, 2021
Contact name Jan Torben Schille
Organization name University of Veterinary Medicine Hannover
Department Small Animal Clinic
Street address Buenteweg 9
City Hannover
ZIP/Postal code 30559
Country Germany
Platforms (2)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL21400 Illumina NextSeq 500 (Canis lupus familiaris)
Samples (27)
GSM4962583 PC-3 cell line - control rep1
GSM4962584 PC-3 cell line - control rep2
GSM4962585 PC-3 cell line - control rep3
BioProject PRJNA683222
SRA SRP297007

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Supplementary file Size Download File type/resource
GSE162832_CT1258_12h_vs_control.xlsx 1.4 Mb (ftp)(http) XLSX
GSE162832_CT1258_24h_vs_control.xlsx 1.4 Mb (ftp)(http) XLSX
GSE162832_CT1258_gene_counts.csv.gz 230.9 Kb (ftp)(http) CSV
GSE162832_LNCaP_12h_vs_control.xlsx 1.8 Mb (ftp)(http) XLSX
GSE162832_LNCaP_24h_vs_control.xlsx 1.8 Mb (ftp)(http) XLSX
GSE162832_LNCaP_gene_counts.csv.gz 320.5 Kb (ftp)(http) CSV
GSE162832_PC-3_12h_vs_control.xlsx 1.6 Mb (ftp)(http) XLSX
GSE162832_PC-3_24h_vs_control.xlsx 1.9 Mb (ftp)(http) XLSX
GSE162832_PC-3_gene_counts_without_PD1.csv.gz 313.4 Kb (ftp)(http) CSV
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