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Series GSE162705 Query DataSets for GSE162705
Status Public on Jan 02, 2021
Title Impact of short- and long-term extracellular acidosis on gene expression of tumors in vitro and in vivo
Organism Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary Purpose: The aim of the study was to analyze the impact of acidic pHe on gene expression in two experimental tumor lines of the rat in vitro and in vivo. The results of short-term incubation (24 h) were compared to chronically acidosis-adapted cells (5 weeks).
Methods: Gene expression was analyzed in AT1 prostate and Walker-256 mammary carcinoma of the rat. Cells were exposed to pH 7.4 and 6.6 for 24 h or 5 weeks. Experimental tumors were implanted in rats and control tumors were compared to tumors in which the glycolytic metabolism was intensified.
Results: The analyses revealed that 147 genes were uniformly regulated in both cell lines (in vitro) and 79 genes in both experimental tumors after 24 h at low pH. 265 genes were uniformly regulated during long- and short-term exposure to low pH.
Overall design Exposure of two different tumor cell lines to pH 7.4 and 6.6 in cell culture and solid experimental tumors.
Adaptation of one tumor cell line to pH 6.6 of 11 passages (=5 weeks).
Contributor(s) Rauschner M, Riemann A, Reime S, Nolze A, Thews O
Citation(s) 33407762
Submission date Dec 04, 2020
Last update date Apr 06, 2021
Contact name Oliver Thews
Phone +49-345-5574048
Organization name University of Halle
Department Institute of Physiology
Street address Magdeburger Str. 6
City Halle/S.
ZIP/Postal code 06112
Country Germany
Platforms (2)
GPL14844 Illumina HiSeq 2000 (Rattus norvegicus)
GPL17116 Illumina HiScanSQ (Rattus norvegicus)
Samples (36)
GSM4957636 AT1 cells pH 7.4 #1
GSM4957637 AT1 cells pH 7.4 #2
GSM4957638 AT1 cells pH 7.4 #3
BioProject PRJNA682631
SRA SRP295990

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Supplementary file Size Download File type/resource
GSE162705_AT1_W256_cells_short-term_acidosis.csv.gz 700.6 Kb (ftp)(http) CSV
GSE162705_AT1_W256_tumors_short-term_acidosis.csv.gz 847.0 Kb (ftp)(http) CSV
GSE162705_AT1_cells_long-term_acidosis.csv.gz 556.8 Kb (ftp)(http) CSV
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