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Status |
Public on Dec 05, 2020 |
Title |
Epigenetic traits inscribed in chromatin accessibility in aged hematopoietic stem cells |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing Genome binding/occupancy profiling by high throughput sequencing
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Summary |
This SuperSeries is composed of the SubSeries listed below.
Hematopoietic stem cells (HSCs) exhibit considerable cell-intrinsic changes with age. Here, we performed an integrated analysis of transcriptome and chromatin accessibility of aged HSCs and downstream progenitors. Alterations in chromatin accessibility preferentially took place in HSCs with aging, which gradually resolved with differentiation. Differentially open accessible regions (open DARs) in aged HSCs showed enrichment of binding motifs of the STAT, ATF, and CNC family transcription factors that are activated in response to external stresses, and most of them comprised active, primed , and inactive enhancers. Genes linked to open DARs showed significantly higher levels of basal expression and their expression reached significantly higher peaks after cytokine stimulation in aged HSCs than in young HSCs, suggesting that open DARs contribute to augmented transcriptional responses under stress conditions. These results indicate that the ongoing and/or history of exposure to external stresses is epigenetically inscribed in HSCs to augment their responses to external stimuli.
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Overall design |
Refer to individual Series
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Citation(s) |
35577813 |
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Submission date |
Dec 04, 2020 |
Last update date |
May 17, 2022 |
Contact name |
Atsushi Iwama |
Organization name |
The Institute of Medical Science, The University of Tokyo
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Department |
Center for Stem Cell Biology and Regenerative Medicine
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Lab |
Division of Stem Cell and Molecular Medicine
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Street address |
4-6-1, Shirokanedai
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City |
Minato-ku |
State/province |
Tokyo |
ZIP/Postal code |
108-8639 |
Country |
Japan |
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Platforms (1) |
GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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Samples (123)
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This SuperSeries is composed of the following SubSeries:
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GSE162551 |
Epigenetic traits inscribed in chromatin accessibility in aged hematopoietic stem cells(ATAC-seq) |
GSE162570 |
Epigenetic traits inscribed in chromatin accessibility in aged hematopoietic stem cells (ChIP-seq H3K27me3 H3K4me3) |
GSE162607 |
Epigenetic traits inscribed in chromatin accessibility in aged hematopoietic stem cells (RNA-seq) |
GSE162659 |
Epigenetic traits inscribed in chromatin accessibility in aged hematopoietic stem cells (EZH2_KO_HSC) |
GSE169206 |
Epigenetic traits inscribed in chromatin accessibility in aged hematopoietic stem cells (RNA-seq of HSCs after cytokine stimulation) |
GSE190419 |
Epigenetic traits inscribed in chromatin accessibility in aged hematopoietic stem cells(ATAC-seq in cytokine and LPS:polyIpolyC experiments)) |
GSE190420 |
Epigenetic traits inscribed in chromatin accessibility in aged hematopoietic stem cells (CUT&TAG H3K27ac,H3K4me1) |
GSE190422 |
Epigenetic traits inscribed in chromatin accessibility in aged hematopoietic stem cells (RNA-seq of MPPs after cytokine stimulation) |
GSE190424 |
Epigenetic traits inscribed in chromatin accessibility in aged hematopoietic stem cells (single cell ATAC-seq) |
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Relations |
BioProject |
PRJNA682581 |