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| Status |
Public on Sep 20, 2021 |
| Title |
Lower brown adipose tissue activity is associated with non-alcoholic fatty liver disease but not changes in the gut microbiota |
| Organisms |
feces metagenome; human feces metagenome |
| Experiment type |
Other
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| Summary |
In rodents, brown adipose tissue (BAT) contributes to whole body energy expenditure and low BAT activity is related to hepatic fat accumulation, partially attributable to the gut microbiome. Little is known of these relationships in humans. In adults (n=60), we assessed hepatic fat and cold-stimulated BAT activity utilizing magnetic resonance imaging and the gut microbiome with 16S sequencing. We transplanted gnotobiotic mice with feces from humans to assess the transferability of BAT activity and NAFLD through the microbiome. Individuals with NAFLD (n=29) had lower BAT activity than those without and BAT activity was inversely related to hepatic fat. Although the fecal microbiome was different in those with NAFLD, no differences were observed in relation to BAT activity and neither of these phenotypic traits were transmissible through fecal transplant to gnotobiotic mice. Thus, low BAT activity is associated with hepatic steatosis but this is not mediated through the gut microbiota.
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| Overall design |
60 stool samples from human adults. For the microbial transfer to mice; we used 9 NAFLD-/H-BAT + and 13 NAFLD+/L-BAT-.
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| Contributor(s) |
Ahmed BA, Ong FJ, Barra NG, Blondin DP, Gunn E, Oreskovich SM, Szamosi JC, Syed SA, Hutchings EK, Konyer NB, Singh NP, Yabut JM, Desjardins EM, Anhê FF, Foley KP, Holloway AC, Noseworthy MD, Haman F, Carpentier AC, Surette MG, Schertzer JD, Punthakee Z, Steinberg GR, Morrison KM |
| Citation(s) |
34622234 |
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| Submission date |
Dec 03, 2020 |
| Last update date |
Dec 20, 2021 |
| Contact name |
Katherine Morrison |
| Organization name |
McMaster
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| Street address |
1280 Main street west
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| City |
Hamilton |
| ZIP/Postal code |
L8S 4L8 |
| Country |
Canada |
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| Platforms (2) |
| GPL24116 |
Illumina MiSeq (feces metagenome) |
| GPL29470 |
Illumina MiSeq (human feces metagenome) |
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| Samples (82)
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| Relations |
| BioProject |
PRJNA682382 |
| SRA |
SRP295668 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE162608_ASV_humans_updated.csv.gz |
76.9 Kb |
(ftp)(http) |
CSV |
| GSE162608_ASV_mice_JDS122-133.csv.gz |
46.8 Kb |
(ftp)(http) |
CSV |
| GSE162608_ASV_mice_JDS539-548.csv.gz |
38.7 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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