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| Status |
Public on Nov 01, 2022 |
| Title |
The cellular basis for epithelial-mesenchymal interaction during mouse tooth development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In this study, using mouse molar as the model, we developed a dual fluorescence reporter mouse to precisely track and analyze dental epithelium and mesenchyme at single-cell resolution from early embryonic to postnatal stages. Moreover, we constructed the virtual molar explorer (VMEx) to spatially map 15,967 molar-expressed genes and identified that Msx1+ Sdc1+ marked the developing dental papilla while surrounded by Msx1+ Sdc1- molar niche. Through tooth germ reconstitution and organoid culture in vitro and kidney capsule transplantation in vivo, we provided evidence that the Msx1+ Sdc1- dental follicle cells might function as the tooth organizers that promoted epithelium survival and tooth germ organization. Furthermore, the appearance of Msx1+ Sdc1+ dental papilla cells relied on the interaction between dental epithelium and Msx1+ Sdc1- dental follicle cells. Together, our results revealed the cellular dynamics of tooth development in mice and identified that the dental follicle might be the key driver of epithelial-mesenchymal interaction and tooth morphogenesis.
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| Overall design |
Tooth germs were dissected and collected at different development stages, including bud stage (E12.5), cap stage (E14.5), bell stage (E16.5), postnatal day 1 (PN1) and day 7 (PN7). Epithelial and mesenchymal cells were enriched by FACS sorting using copGFP and tdTomato fluorescence. Cells were then used for single-cell RNA sequencing using 10X Genomics platform.
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| Contributor(s) |
Duan Y, Fu H, Hu H, Liao Y, Kang F, Zhang Z, Zhang B, Huo F, Yin Y, Chen G, Hu H, Tian W, Li Z |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Dec 01, 2020 |
| Last update date |
Nov 03, 2022 |
| Contact name |
Zhonghan Li |
| E-mail(s) |
zhonghan.li@scu.edu.cn
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| Phone |
+86-28-85463752
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| Organization name |
Sichuan University
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| Department |
College of Life Science
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| Street address |
29 Wangjiang Road
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| City |
Chengdu |
| State/province |
Sichuan |
| ZIP/Postal code |
610064 |
| Country |
China |
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| Platforms (2) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA681820 |
| SRA |
SRP295338 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE162413_RAW.tar |
1006.2 Mb |
(http)(custom) |
TAR (of TAR, ZIP) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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