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| Status |
Public on Jul 17, 2021 |
| Title |
Satellite cell depletion results in a cryptic subpopulation of myonuclei in response to exercise |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Skeletal muscle is composed of post-mitotic myofibers that form a syncytium containing hundreds of myonuclei. Using a progressive exercise training model in the mouse and single nucleus RNA-sequencing (snRNA-seq) for high resolution characterization of myonuclear transcription, we show myonuclear functional specialization in muscle from sedentary mice that is altered by 4 weeks of exercise training. Furthermore, in response to exercise, snRNA-seq reveals that resident muscle stem cells, satellite cells, were activated, but demonstrated limited lineage progression while contributing to muscle adaption. In the absence of satellite cells, trajectory analysis predicts the emergence of cryptic myonuclei that appear to lose their canonical identity. These data illustrate how myonuclear adaptation to exercise may be disrupted under conditions satellite cell compromise.
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| Overall design |
We used a genetic mouse model to fluorescently label and purify myonuclei from skeleletal muscle to investigate single-myonuclear transcriptomes using single-nucleus RNA-sequencing. Additionally, we used a translatable model of exercise training in conjunction with a mouse model of inducible satellite cell (skeletal muscle precursor cells) ablation to study single-nuclear transcriptomic changes after 4-weeks of exercise training at 4 and 24 hours after the last bout of exercise.
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| Contributor(s) |
Wen Y, Englund DA, McCarthy JJ, Peterson CA |
| Citation(s) |
34368654 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AR060701 |
Novel Roles for Satellite Cells in Adult Skeletal Muscle Adaptation |
UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION |
JOHN Joseph MCCARTHY |
| R01 AG049806 |
The effects of exercise on satellite cell dynamics during aging |
UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION |
CHARLOTTE A. PETERSON |
| F32 AR071753 |
Fusion-Independent Roles of Satellite Cells in Muscle Adaptation |
UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION |
Kevin Murach |
| K99 AG063994 |
Myonuclear Epigenetics of Skeletal Muscle Mass Regulation with Age |
UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION |
Kevin Murach |
| F31 AR075364 |
Determining the fiber type-specific requirements for satellite cells during skeletal muscle hypertrophy |
UNIVERSITY OF KENTUCKY RESEARCH FOUNDATION |
Davis A. Englund |
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| Submission date |
Nov 29, 2020 |
| Last update date |
Aug 11, 2021 |
| Contact name |
Yuan Wen |
| E-mail(s) |
ywen2@g.uky.edu
|
| Phone |
8592186846
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| Organization name |
University of Kentucky
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| Street address |
760 Press Ave
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| City |
Lexington |
| State/province |
Kentucky |
| ZIP/Postal code |
40536 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA681403 |
| SRA |
SRP294967 |
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