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Series GSE162234 Query DataSets for GSE162234
Status Public on Feb 25, 2021
Title Time-resolved single cell transcriptomics of an ibrutinib-treated mantle cell lymphoma cell line
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We performed a high-throughput droplet-based single-cell RNA sequencing experiment on a sensitive MCL cell line model (REC-1) treated with ibrutinib over time using the 10x Genomics platform. Two biological replicates were generated of an untreated control, 6 h, and 48 h ibrutinib-treated cells.
Overall design REC-1 cells were cultured for 48 h, one sample stayed untreated, the second was treated with 400 nM ibrutinib for 6 h and the third for 48 h. After harvesting, the cells were stained with propidium iodide to identify the surviving cells which entered single-cell RNA sequencing.
Contributor(s) Fuhr V, Vafadarnejad E, Dietrich O, Arampatzi P, Riedel A, Saliba AE, Rosenwald A, Rauert-Wunderlich H
Citation(s) 33668876
Submission date Nov 27, 2020
Last update date Mar 08, 2021
Contact name Antoine-Emmanuel Saliba
Phone +49-931-31-81341
Organization name Helmholtz Institute for RNA-based Infection Research
Street address Josef-Schneider-Straße 2 / D15
City Würzburg
ZIP/Postal code 97080
Country Germany
Platforms (1)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (6)
GSM4947350 REC-1 cells, F2
GSM4947351 REC-1 cells, F3
GSM4947352 REC-1 cells, F4
BioProject PRJNA681209
SRA SRP294496

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE162234_RAW.tar 3.1 Gb (http)(custom) TAR (of H5, TAR)
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Raw data are available in SRA
Processed data provided as supplementary file

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