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Series GSE161864 Query DataSets for GSE161864
Status Public on Feb 21, 2022
Title WNT11/ROR2 signaling is associated with tumor invasion and poor survival in breast cancer
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Breast cancer has been associated with activation of the WNT signaling pathway, although the underlying molecular mechanisms are still unclear. In order to understand the molecular basis of these observations, we overexpressed ROR2 in human breast cancer cell lines and characterized them by RNA-Sequencing. High levels of ROR2 were associated with defects in cell morphology and cell-cell-contacts leading to increased tumor invasiveness. Using gene expression analysis we demonstrated an upregulation of several non-canonical WNT ligands in ROR2-overexpressing breast cancer cells, in particular WNT11. Knockdown of WNT11 reversed the pro-invasive phenotype and the cellular changes in ROR2-overexpressing cells. Taken together, our studies revealed a novel auto-stimulatory loop in which ROR2 triggers the expression of its own ligands, e.g. WNT11, resulting in enhanced tumor invasion associated with breast cancer metastasis.
Overall design Human breast cancer cells (MCF-7, SK-BR-3 and BT-474) were transfected either with an empty vector (pcDNA) or with a hROR2 overexpression plasmid (pROR2). To gain further insight into additional pathways regulated specifically by ROR2/WNT11, we characterized ROR2-overexpressing MCF-7 cells with siRNA-mediated WNT11 knockdown by RNA-Seq.
Contributor(s) Menck K, Heinrichs S, Wlochowitz D, Sitte M, Noeding H, Janshoff A, Treiber H, Ruhwedel T, Schatlo B, von der Brelie C, Wiemann S, Pukrop T, Beißbarth T, Binder C, Bleckmann A
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Submission date Nov 20, 2020
Last update date Mar 12, 2022
Contact name Darius Wlochowitz
Organization name Institute of Bioinformatics
Department Bioinformatics
Street address Goldschmidtstr. 1
City Göttingen
ZIP/Postal code 37077
Country Germany
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL30173 NextSeq 2000 (Homo sapiens)
Samples (27)
GSM4916163 mcf7_pcDNA_wt_1
GSM4916164 mcf7_pcDNA_wt_2
GSM4916165 mcf7_pcDNA_wt_3
This SubSeries is part of SuperSeries:
GSE161866 WNT11 is a novel ligand for ROR2 in human breast cancer
BioProject PRJNA679736
SRA SRP293436

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE161864_RNA_Seq_RSEM_count_MCF7_WNT11_ROR2.csv.gz 1.2 Mb (ftp)(http) CSV
GSE161864_RNA_Seq_featureCount_counts_BT474_SKBR3.csv.gz 589.7 Kb (ftp)(http) CSV
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Processed data are available on Series record

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