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Series GSE161693 Query DataSets for GSE161693
Status Public on Jan 07, 2022
Title Notch2 deficiency prevents muscle atrophy induced by mechanical unloading and diabetes.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Notch signaling plays essential roles in maintenance of muscle stem cell pool. We found that Notch2, but not Notch1 and Notch3, is expressed in fully differentiated myofibers. To study the specific role of Notch2 in adult myofibers, we generated muscle-specific Notch2-knockout mice. Here, we showed that muscle-specific Notch2 deficiency prevented muscle atrophy induced by hindlimb unloading and diabetes millitus. RNA sequencing analysis revealed that the loss of Notch2 gene in myofibers inhibited gene responses to unloading and diabetes. Especially, several FoxO-target genes and atrogenes were upregulated in wildtype muscles but not in Notch2-deficient muscles by unloading and diabetes. Thus, our characterization of muscle-specific Notch2-knockout mice indicates that Notch2 acts as a regulatory factor of skeletal muscle plasticity and could be a therapeutic target of muscle atrophy.
 
Overall design mRNA profiles of gastrocnemius muscle tissues obtained from WT and Notch2-mKO mice under unloading, diabetic, or control condition.
 
Contributor(s) Fujimaki S, Ono Y
Citation(s) 35228746
Submission date Nov 18, 2020
Last update date Apr 08, 2022
Contact name Shin Fujimaki
E-mail(s) shin.fujimaki0526@gmail.com
Organization name Kumamoto University
Department IMEG
Lab Muscle Development & Regeneration
Street address Honjo 2-2-1
City Kumamoto
ZIP/Postal code 8600811
Country Japan
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (24)
GSM4912796 WT-Control rep1
GSM4912797 WT-Control rep2
GSM4912798 WT-Control rep3
Relations
BioProject PRJNA679176
SRA SRP293052

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE161693_N2-mKO_Control-Diabetes.txt.gz 2.3 Mb (ftp)(http) TXT
GSE161693_N2-mKO_Control-Unloading.txt.gz 2.3 Mb (ftp)(http) TXT
GSE161693_WT_Control-Diabetes.txt.gz 2.3 Mb (ftp)(http) TXT
GSE161693_WT_Control-Unloading.txt.gz 2.3 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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