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Series GSE161596 Query DataSets for GSE161596
Status Public on Jan 04, 2021
Title Deletion of the helicase RTEL1 in RNA:DNA hybrids throughout the genome and how this may be affected by overexpression of the protein WT RNaseH1GFP in MEFs
Organism Mus musculus
Experiment type Other
Summary The goal of this study is to assess the effect of the deletion of the helicase RTEL1 in RNA:DNA hybrids throughout the genome and how this may be affected by overexpression of the protein WT RNaseH1GFP in mouse embryonic fibroblasts.
 
Overall design We used RTEL1F/F mouse embryonic fibroblasts that upon infection with Cre adenoparticles delete Rtel1 and upon addition of doxycycline overexpress WT RNaseH1GFP (Rtel1F/F;WT RNH1-GFP MEFs). Rtel1F/F;WT RNH1-GFP MEFs were infected with GFP or Cre-GFP adenovirus, after 48 h doxycycline (2 μg/ml) was added and cells were collected after 48 h. RNA:DNA hybrids were pulled down using the DRIP protocol and DNA was isolated and send for sequencing.
 
Contributor(s) Margalef P, Kotsantis P, Segura-Bayona S, Poetsch AR, Patel H, Ruis P, Marzec P, Bellelli R, Hewitt G, Stanage TH, Goldstone R, Boulton SJ
Citation(s) 33357438
Submission date Nov 17, 2020
Last update date Jan 04, 2021
Contact name Simon J Boulton
E-mail(s) simon.boulton@crick.ac.uk
Organization name The Francis Crick Institute
Lab DSB Repair Metabolism Lab
Street address 1 Midland Rd
City London
ZIP/Postal code NW1 1AT
Country United Kingdom
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (8)
GSM4910678 Rtel1f/f Ad-Cre +DOX DRIP biological replicate 1
GSM4910679 Rtel1f/f Ad-Cre MOCK DRIP biological replicate 1
GSM4910680 Rtel1f/f Ad-GFP +DOX DRIP biological replicate 1
This SubSeries is part of SuperSeries:
GSE161597 RTEL1 regulates G4/R-loops to avert replication-transcription collisions
Relations
BioProject PRJNA678869
SRA SRP292869

Download family Format
SOFT formatted family file(s) SOFTHelp
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Supplementary file Size Download File type/resource
GSE161596_RAW.tar 2.0 Gb (http)(custom) TAR (of BIGWIG, BROADPEAK)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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