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Status |
Public on Jan 04, 2021 |
Title |
Deletion of the helicase RTEL1 in RNA:DNA hybrids throughout the genome and how this may be affected by overexpression of the protein WT RNaseH1GFP in MEFs |
Organism |
Mus musculus |
Experiment type |
Other
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Summary |
The goal of this study is to assess the effect of the deletion of the helicase RTEL1 in RNA:DNA hybrids throughout the genome and how this may be affected by overexpression of the protein WT RNaseH1GFP in mouse embryonic fibroblasts.
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Overall design |
We used RTEL1F/F mouse embryonic fibroblasts that upon infection with Cre adenoparticles delete Rtel1 and upon addition of doxycycline overexpress WT RNaseH1GFP (Rtel1F/F;WT RNH1-GFP MEFs). Rtel1F/F;WT RNH1-GFP MEFs were infected with GFP or Cre-GFP adenovirus, after 48 h doxycycline (2 μg/ml) was added and cells were collected after 48 h. RNA:DNA hybrids were pulled down using the DRIP protocol and DNA was isolated and send for sequencing.
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Contributor(s) |
Margalef P, Kotsantis P, Segura-Bayona S, Poetsch AR, Patel H, Ruis P, Marzec P, Bellelli R, Hewitt G, Stanage TH, Goldstone R, Boulton SJ |
Citation(s) |
33357438 |
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Submission date |
Nov 17, 2020 |
Last update date |
Jan 04, 2021 |
Contact name |
Simon J Boulton |
E-mail(s) |
simon.boulton@crick.ac.uk
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Organization name |
The Francis Crick Institute
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Lab |
DSB Repair Metabolism Lab
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Street address |
1 Midland Rd
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City |
London |
ZIP/Postal code |
NW1 1AT |
Country |
United Kingdom |
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Platforms (1) |
GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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Samples (8)
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GSM4910678 |
Rtel1f/f Ad-Cre +DOX DRIP biological replicate 1 |
GSM4910679 |
Rtel1f/f Ad-Cre MOCK DRIP biological replicate 1 |
GSM4910680 |
Rtel1f/f Ad-GFP +DOX DRIP biological replicate 1 |
GSM4910681 |
Rtel1f/f Ad-GFP MOCK DRIP biological replicate 1 |
GSM4910682 |
Rtel1f/f Ad-Cre +DOX Input biological replicate 1 |
GSM4910683 |
Rtel1f/f Ad-Cre MOCK Input biological replicate 1 |
GSM4910684 |
Rtel1f/f Ad-GFP +DOX Input biological replicate 1 |
GSM4910685 |
Rtel1f/f Ad-GFP MOCK Input biological replicate 1 |
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This SubSeries is part of SuperSeries: |
GSE161597 |
RTEL1 regulates G4/R-loops to avert replication-transcription collisions |
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Relations |
BioProject |
PRJNA678869 |
SRA |
SRP292869 |