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Series GSE16146 Query DataSets for GSE16146
Status Public on May 18, 2010
Title The response of Mycobacterium tuberculosis to reactive oxygen and nitrogen species
Organism Mycobacterium tuberculosis
Experiment type Expression profiling by array
Summary The bacteriostatic and bactericidal effects and the corresponding expression profiles of Mycobacterium tuberculosis to representative oxidative and nitrosative stresses were investigated by growth and survival studies and whole genome expression analysis. The response of M. tuberculosis to a range of hydrogen peroxide (H2O2) concentrations tended to fall into three distinct categories: (1) low level exposure resulted in induction of few H2O2 sensitive genes, (2) intermediate exposure resulted in massive transcriptional changes without an effect on growth or survival, and (3) high exposure resulted in a muted transcriptional response and eventual death. Nitric oxide (NO) exposure initiated much of the same transcriptional response as H2O2. However, unlike H2O2 exposure, NO exposure affected a dose-dependent bacteriostatic activity without killing and induction of dormancy-related genes. Included in the shared response to H2O2 and NO was the induction of genes encoding oxidative stress detoxification and iron-sulfur cluster repair functions. Expression of several key oxidative stress defense genes was constitutive, or increased moderately from an already elevated level, suggesting bacilli that are continually primed for oxidative stress defense. Deletion of the known oxidative stress responsive regulator, FurA, resulted in the constitutive expression of furA, katG, and Rv1907c; while other genes do not appear to be solely controlled by FurA. In contrast to Escherichia coli, M. tuberculosis appears highly resistant to DNA damage-dependent killing caused by low mill molar levels of H2O2. Furthermore, instead of limiting access to iron to prevent hydroxyl radical formation from H2O2 and thus DNA damage, M. tuberculosis induced iron uptake genes in response to H2O2 and NO.
 
Overall design Set of arrays that are part of repeated experiments
Compound Based Treatment: H2O2 or DETA/NO treatment
 
Contributor(s) Voskuil M, Visconti K
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Submission date May 18, 2009
Last update date Mar 21, 2012
Contact name Martin I Voskuil
E-mail(s) martin.voskuil@ucdenver.edu
Phone 303-724-4219
Organization name University of Colorado Denver
Department Microbiology
Lab Voskuil Lab
Street address 12800 E. 19th Ave
City Aurora
State/province CO
ZIP/Postal code 80045
Country USA
 
Platforms (3)
GPL8523 SMD Print_745 Mycobacterium tuberculosis
GPL8561 SMD Print_498 Mycobacterium tuberculosis
GPL8562 SMD Print_580 Mycobacterium tuberculosis
Samples (80)
GSM404398 MTB strain 1254 Ctrl vs 10.0 mM H202 40min rep 5
GSM404399 MTB strain 1254 Ctrl vs 0.5 mM H202 40min rep 1
GSM404400 MTB strain 1254 Ctrl vs 0.5 mM DETA/NO 4hrs rep 3
Relations
BioProject PRJNA115051

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE16146_RAW.tar 290.0 Kb (http)(custom) TAR
Processed data included within Sample table

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