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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 12, 2021 |
| Title |
scRNA-seq of E18.5 small intestinal innate lymphoid cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Embryonic innate lymphoid cells (ILCs) were isolated from small intestinal lamina propria to study their transcriptomic profile in the developing tissue.
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| Overall design |
ILCs were isolated from E18.5 small instestines of Rorc(gt)GFP/wt, Rorc(gt)GFP/GFP and Rorc(gt)GFP/GFP x Tbx21-/- (Hashtagged) and FACS-sorted as viable Lineage(CD19, CD3, CD5, F4/80,FceRIa, Gr-1)-CD45+CD122+ and/or CD127+ combined with the following 25 CITE-Seq antibodies: CD49b, NK1.1, DNAM1, CD3, CCR6, NKp46, CD117, Nrp-1, NKG2D, TCRgd, TCRb, CD49a, CXCR5,CD27, CD200R1, CD4, PD-1, CD127 PE + anti PE, a4b7 + anti-Biotin, ICOS, Sca-1, ST2, CD25, KLRG1
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| Contributor(s) |
Stehle C, Rückert T, Fiancette R, Gajdasik DW, Willis C, Ulbricht C, Durek P, Mashreghi M, Finke D, Hauser AE, Withers DR, Chang H, Zimmermann J, Romagnani C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Nov 13, 2020 |
| Last update date |
Aug 14, 2021 |
| Contact name |
Pawel Durek |
| E-mail(s) |
pawel.durek@drfz.de
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| Organization name |
Deutsches Rheuma-Forschungszentrum
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| Street address |
Charitéplatz 1
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| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA678307 |
| SRA |
SRP292509 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE161441_RAW.tar |
26.9 Mb |
(http)(custom) |
TAR (of H5, TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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