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Series GSE16123 Query DataSets for GSE16123
Status Public on Mar 29, 2010
Title Multiomics study to identify virulence factors of Rickettsia prowazekii revealed its adaptive mutation capabilities
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We identified four virulence phenotypes of Rickettsia prowazekii (the deadly agent of epidemic typhus) that are associated with the upregulation of antiapoptotic genes (virulent strain) or the Interferon I pathway (avirulent). Transcriptional and proteomic analyses of R. prowazekii linked surface protein expression and methylation with virulence. By sequencing a virulent strain and using comparative genomics, we found hotspots of mutations in homopolymeric tracts of poly(A) and poly(T) that lead to gene split and inactivation and explain the loss of virulence in the vaccine strain. These areas of instability explains adaptive mutations leading to virulence recovery in the vaccine strain.
Overall design Several approaches are combined including the transcriptome of endothelial cells (the target cells of Rickettsia prowazekii) in order to identify factors associated with virulence of Rickettsia prowazekii, the agent of epidemic typhus
Contributor(s) Bechah Y, El Karkouri K, Mediannikov O, Leroy Q, Pelletier N, Robert C, Médigue C, Mege J, Raoult D
Citation(s) 20368341
Submission date May 15, 2009
Last update date Feb 22, 2018
Contact name Yassina Bechah
Organization name CNRS
Street address 27 bd J. Moulin
City Marseille
ZIP/Postal code 13005
Country France
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (16)
GSM403281 array controle 1
GSM403377 array controle 2
GSM403378 array controle 3
BioProject PRJNA115011

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE16123_RAW.tar 112.1 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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