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Series GSE161071 Query DataSets for GSE161071
Status Public on Nov 07, 2022
Title cAMP and mGluR1/5 dependent expression of mRNA, lncRNA, and miRNA in mouse hippocampal neurons
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary To identify cAMP-dependent and mGluR1/5-dependent RNAs, we treated DIV16 primary hippocampal neurons with 50uM Forskolin, 30uM DHPG, or equal volume DMSO for 30 minutes prior to RNA isolation using Trizol and Total/small RNA sequencing. We submitted samples to Scripps Florida Genomics Core for library preparation and sequencing.
 
Overall design Hippocampi were cultured from P0 mice for generating RNA at DIV 16. We treated DIV16 primary hippocampal neurons with 50uM Forskolin, 30uM DHPG, or equal volume DMSO for 30 minutes.
 
Contributor(s) Grinman E, Puthanveettil S
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Submission date Nov 09, 2020
Last update date Nov 07, 2022
Contact name Sathyanarayanan V Puthanveettil
E-mail(s) sputhanv@scripps.edu
Phone 561-228-2243
Organization name Scripps Florida
Department Neuroscience
Lab Puthanveettil
Street address 130 Scripps Way
City Jupiter
State/province Florida
ZIP/Postal code 33458
Country USA
 
Platforms (2)
GPL16417 Illumina MiSeq (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (32)
GSM4889192 DMSO_Total_1
GSM4889193 DMSO_Total_2
GSM4889194 DMSO_Total_3
Relations
BioProject PRJNA675483
SRA SRP291690

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE161071_Total_RNA_Counts.csv.gz 1.2 Mb (ftp)(http) CSV
GSE161071_Total_RNA_DHPGvsDMSO.csv.gz 1.1 Mb (ftp)(http) CSV
GSE161071_Total_RNA_FSKvsDMSO.csv.gz 1.1 Mb (ftp)(http) CSV
GSE161071_miRNA_Counts.csv.gz 32.2 Kb (ftp)(http) CSV
GSE161071_miRNA_DHPGvsDMSO.csv.gz 43.2 Kb (ftp)(http) CSV
GSE161071_miRNA_FSKvsDMSO.csv.gz 42.1 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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