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Status |
Public on Nov 07, 2022 |
Title |
cAMP and mGluR1/5 dependent expression of mRNA, lncRNA, and miRNA in mouse hippocampal neurons |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing Non-coding RNA profiling by high throughput sequencing
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Summary |
To identify cAMP-dependent and mGluR1/5-dependent RNAs, we treated DIV16 primary hippocampal neurons with 50uM Forskolin, 30uM DHPG, or equal volume DMSO for 30 minutes prior to RNA isolation using Trizol and Total/small RNA sequencing. We submitted samples to Scripps Florida Genomics Core for library preparation and sequencing.
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Overall design |
Hippocampi were cultured from P0 mice for generating RNA at DIV 16. We treated DIV16 primary hippocampal neurons with 50uM Forskolin, 30uM DHPG, or equal volume DMSO for 30 minutes.
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Contributor(s) |
Grinman E, Puthanveettil S |
Citation missing |
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Submission date |
Nov 09, 2020 |
Last update date |
Nov 07, 2022 |
Contact name |
Sathyanarayanan V Puthanveettil |
E-mail(s) |
sputhanv@scripps.edu
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Phone |
561-228-2243
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Organization name |
Scripps Florida
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Department |
Neuroscience
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Lab |
Puthanveettil
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Street address |
130 Scripps Way
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City |
Jupiter |
State/province |
Florida |
ZIP/Postal code |
33458 |
Country |
USA |
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Platforms (2) |
GPL16417 |
Illumina MiSeq (Mus musculus) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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Samples (32)
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Relations |
BioProject |
PRJNA675483 |
SRA |
SRP291690 |