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Series GSE161055 Query DataSets for GSE161055
Status Public on May 03, 2021
Title Analysis of the transcriptomic changes of myoblasts lacking Hira [RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The epigenetic mechanisms coordinating the maintenance of adult cellular lineages and the inhibition of alternative cell fates are poorly understood. Here we show that targeted ablation of the histone chaperone HIRA in myogenic cells leads to extensive transcriptional modifications consistent with a major role in maintaining skeletal muscle cellular identity. Conditional ablation of HIRA in muscle stem cells of adult mice compromised their capacity to regenerate and self-renew, leading to tissue repair failure. Epigenetic analysis of Hira-deficient cells showed a drastic reduction of histone variant H3.3 deposition and H3K27ac modification at regulatory regions of muscle genes. By contrast, genes from alternative lineages were ectopically expressed in Hira-mutant cells via MLL1/MLL2-mediated increase of H3K4me3 mark at silent promoter regions. Therefore, HIRA sustains the epigenetic landscape governing muscle cell lineage identity via incorporation of H3.3 at muscle gene regulatory regions, while preventing the expression of alternative lineage genes.
 
Overall design Examining the transcriptomic changes in proliferating myoblasts lacking Hira
 
Contributor(s) Esteves de Lima J, Akar RB, Machado L, Li Y, Drayton-Libotte B, Dilworth FJ, Relaix F
Citation(s) 34103504
Submission date Nov 08, 2020
Last update date Jun 15, 2021
Contact name Joana Esteves de Lima
E-mail(s) joanaesteveslima@gmail.com
Organization name INSERM
Department IMRB INSERM U955
Lab Team RELAIX
Street address 8 rue du general sarrail
City Creteil
ZIP/Postal code 94000
Country France
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (6)
GSM4889025 C2C12_rep1
GSM4889026 C2C12_rep2
GSM4889027 C2C12_rep3
This SubSeries is part of SuperSeries:
GSE161056 ATAC-seq, ChIP-seq and RNA-seq studies in myoblasts lacking Hira
Relations
BioProject PRJNA675386
SRA SRP291645

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE161055_DESeq2_C2C12vsHiraKO.txt.gz 1.6 Mb (ftp)(http) TXT
GSE161055_Normalized_counts_file_C2C12vsHiraKO.txt.gz 1.2 Mb (ftp)(http) TXT
GSE161055_RAW.tar 1.4 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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