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Status |
Public on May 03, 2021 |
Title |
Analysis of the transcriptomic changes of myoblasts lacking Hira [RNA-seq] |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
|
Summary |
The epigenetic mechanisms coordinating the maintenance of adult cellular lineages and the inhibition of alternative cell fates are poorly understood. Here we show that targeted ablation of the histone chaperone HIRA in myogenic cells leads to extensive transcriptional modifications consistent with a major role in maintaining skeletal muscle cellular identity. Conditional ablation of HIRA in muscle stem cells of adult mice compromised their capacity to regenerate and self-renew, leading to tissue repair failure. Epigenetic analysis of Hira-deficient cells showed a drastic reduction of histone variant H3.3 deposition and H3K27ac modification at regulatory regions of muscle genes. By contrast, genes from alternative lineages were ectopically expressed in Hira-mutant cells via MLL1/MLL2-mediated increase of H3K4me3 mark at silent promoter regions. Therefore, HIRA sustains the epigenetic landscape governing muscle cell lineage identity via incorporation of H3.3 at muscle gene regulatory regions, while preventing the expression of alternative lineage genes.
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Overall design |
Examining the transcriptomic changes in proliferating myoblasts lacking Hira
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Contributor(s) |
Esteves de Lima J, Akar RB, Machado L, Li Y, Drayton-Libotte B, Dilworth FJ, Relaix F |
Citation(s) |
34103504 |
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Submission date |
Nov 08, 2020 |
Last update date |
Jun 15, 2021 |
Contact name |
Joana Esteves de Lima |
E-mail(s) |
joanaesteveslima@gmail.com
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Organization name |
INSERM
|
Department |
IMRB INSERM U955
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Lab |
Team RELAIX
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Street address |
8 rue du general sarrail
|
City |
Creteil |
ZIP/Postal code |
94000 |
Country |
France |
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Platforms (1) |
GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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Samples (6)
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This SubSeries is part of SuperSeries: |
GSE161056 |
ATAC-seq, ChIP-seq and RNA-seq studies in myoblasts lacking Hira |
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Relations |
BioProject |
PRJNA675386 |
SRA |
SRP291645 |