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Series GSE161053 Query DataSets for GSE161053
Status Public on May 03, 2021
Title Genome-wide changes in chromatin accessibility in myoblasts lacking Hira [ATAC-seq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The epigenetic mechanisms coordinating the maintenance of adult cellular lineages and the inhibition of alternative cell fates are poorly understood. Here we show that targeted ablation of the histone chaperone HIRA in myogenic cells leads to extensive transcriptional modifications consistent with a major role in maintaining skeletal muscle cellular identity. Conditional ablation of HIRA in muscle stem cells of adult mice compromised their capacity to regenerate and self-renew, leading to tissue repair failure. Epigenetic analysis of Hira-deficient cells showed a drastic reduction of histone variant H3.3 deposition and H3K27ac modification at regulatory regions of muscle genes. By contrast, genes from alternative lineages were ectopically expressed in Hira-mutant cells via MLL1/MLL2-mediated increase of H3K4me3 mark at silent promoter regions. Therefore, HIRA sustains the epigenetic landscape governing muscle cell lineage identity via incorporation of H3.3 at muscle gene regulatory regions, while preventing the expression of alternative lineage genes.
 
Overall design Examining the changes in chromatin accessibility in proliferating myoblasts lacking Hira
 
Contributor(s) Esteves de Lima J, Akar RB, Machado L, Li Y, Drayton-Libotte B, Dilworth FJ, Relaix F
Citation(s) 34103504
Submission date Nov 08, 2020
Last update date Jun 15, 2021
Contact name Joana Esteves de Lima
E-mail(s) joanaesteveslima@gmail.com
Organization name INSERM
Department IMRB INSERM U955
Lab Team RELAIX
Street address 8 rue du general sarrail
City Creteil
ZIP/Postal code 94000
Country France
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (4)
GSM4889011 C2C12_ATAC_rep1
GSM4889012 C2C12_ATAC_rep2
GSM4889013 Hira_KO_ATAC_rep1
This SubSeries is part of SuperSeries:
GSE161056 ATAC-seq, ChIP-seq and RNA-seq studies in myoblasts lacking Hira
Relations
BioProject PRJNA675384
SRA SRP291643

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE161053_RAW.tar 781.2 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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