|
| Status |
Public on Oct 05, 2021 |
| Title |
Chromatin accessibility changes in experimentally-induced basal to squamous cell carcinoma transition (BST) |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Basal cell carcinoma may undergo BST spontaneously or upon Hedgehog targeting therapy. We identified that modulation of Ras/MAPK or TGFb signaling drive BST. Here, we induce Ras/MAPK and/or abrogate TGFb signaling to induce BST. Alternatively we drive c-FOS to induce BST. In these various experimentally-induced model of BST, we analyze chromatin accessibility profiles upon Ras/MAPK activation and/or TGFb signaling abrogation. We also analyze chromatin accessibility profiles upon c-FOS activation.
|
| |
|
| Overall design |
RasV12-expressing and/or TGFbR1-kd ASZcells were processed for ATAC-seq. Two biological replicates were sequenced per treatment group.
ATACseq profiles of ASZ transfected with doxycycline-inducible empty vector (ctrl) or c-FOS containing vector (c-FOS) upon Dox treatment, were generated by deep sequencing, in duplicate, using Illumina NextSeq. The sequence reads that passed quality filters were analyzed using bowtie for alignment and macs2 for peak calling.
|
| |
|
| Contributor(s) |
Kuonen F, Gaddam S |
| Citation(s) |
34610301 |
| |
| Submission date |
Nov 06, 2020 |
| Last update date |
Oct 19, 2021 |
| Contact name |
Anthony Oro |
| Organization name |
Stanford University
|
| Department |
Dermatology
|
| Street address |
269 Campus Drvie
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (16)
|
|
| This SubSeries is part of SuperSeries: |
| GSE168376 |
C-FOS-driven basal to squamous cell transition |
|
| Relations |
| BioProject |
PRJNA675105 |
| SRA |
SRP291459 |
Less...
More...