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Status |
Public on Jul 07, 2022 |
Title |
mRNASeq of glioma cells |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
In mouse models of PDGF-driven glioma where Notch is tumor-suppressive, we find that Notch activity shapes interferon-response in tumor cells and the local immune microenvironment early during tumor progression. Notch inhibition disturbs cytokine expression by tumor cells, altering recruitment and activation of immune cell populations and favoring hyper-proliferative "immune niche" independent growth. Genetic inactivation of Notch in glioma cells also attenuates their response to interferon-gamma, establishing a non-cell autonomous inhibitory feedback loop that further promotes immune evasion and aggressiveness. Hence, we propose that Notch signaling levels in tumor cells are key to orchestrate immune escape and microenvironment independency during brain tumor formation.
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Overall design |
Expression profiles of PDGF+Trp53-/- glioma cells from tumors with four different Notch mutant genotypes (Rbpj-/-, Notch1-/-Notch2-/-, Notch1-/-, and Notch2-/-) and tumors with intact Notch signaling (PDGF+Trp53-/- control tumors)
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Contributor(s) |
Ivanek R, Parmigiani E, Taylor V, Giachino C |
Citation missing |
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Submission date |
Nov 02, 2020 |
Last update date |
Jul 07, 2022 |
Contact name |
DBM Bioinformatics Core Facility |
Phone |
+41612073541
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Organization name |
University of Basel
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Department |
Departement of Biomedicine
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Street address |
Hebelstrasse 20
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City |
Basel |
State/province |
BS |
ZIP/Postal code |
4053 |
Country |
Switzerland |
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Platforms (1) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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Samples (26)
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This SubSeries is part of SuperSeries: |
GSE160715 |
Notch activity in tumor cells shapes interferon-gamma response and immune microenvironment in glioma |
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Relations |
BioProject |
PRJNA673900 |
SRA |
SRP290848 |