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Series GSE160668 Query DataSets for GSE160668
Status Public on Jun 27, 2021
Title Analysis on Replication and Transcription of SARS-CoV-2 and inhition effect of Remdesivir
Organisms Chlorocebus sabaeus; Severe acute respiratory syndrome coronavirus 2
Experiment type Expression profiling by high throughput sequencing
Summary SARS-CoV-2, a betacoronavirus with a positive-sense, single-stranded RNA genome, caused the COVID-19 pandemic with unprecedent health and socio-economic crisis. Although its general sense mRNA architecture was reported, that of its negative strand was unexplored. We combined poly(A)-mRNA and ribosomal RNA-depleted sequencing to delineate several fine features of both RNA strands. Together with the transcriptome data under the perturbation of anti-viral drug Remdesivir, this project opens new sights into SARS-CoV-2 replication mechanism and may facilitate the anti-viral drug design.
 
Overall design Overall 10 samples are analyzed, six of which are Poly(A) RNA-seq and four are Ribozero RNA-seq samples. Poly(A) RNA-seq samples contain two treatment conditions with triplicates. Ribozero RNA-seq samples contain two treatment conditions with duplicates.
 
Contributor(s) Hu Y, Zhao J, Zhao Y, Sun J
Citation(s) 34278249
Submission date Nov 02, 2020
Last update date Jul 29, 2021
Contact name Yan Zhao
E-mail(s) 11962001@mail.sustech.edu.cn
Organization name Southern University of Science and Technology
Department BIology
Street address No. 1088,Xueyuandadao
City Shenzhen
ZIP/Postal code 518055
Country China
 
Platforms (1)
GPL29334 Illumina NovaSeq 6000 (Chlorocebus sabaeus; Severe acute respiratory syndrome coronavirus 2)
Samples (10)
GSM4876677 Virus-24hpi-Rep1-VeroE6-PolyA
GSM4876678 Virus-24hpi-Rep2-VeroE6-PolyA
GSM4876679 Virus-24hpi-Rep3-VeroE6-PolyA
Relations
BioProject PRJNA673899
SRA SRP290847

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE160668_RAW.tar 9.2 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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