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Status |
Public on Jun 27, 2021 |
Title |
Analysis on Replication and Transcription of SARS-CoV-2 and inhition effect of Remdesivir |
Organisms |
Chlorocebus sabaeus; Severe acute respiratory syndrome coronavirus 2 |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
SARS-CoV-2, a betacoronavirus with a positive-sense, single-stranded RNA genome, caused the COVID-19 pandemic with unprecedent health and socio-economic crisis. Although its general sense mRNA architecture was reported, that of its negative strand was unexplored. We combined poly(A)-mRNA and ribosomal RNA-depleted sequencing to delineate several fine features of both RNA strands. Together with the transcriptome data under the perturbation of anti-viral drug Remdesivir, this project opens new sights into SARS-CoV-2 replication mechanism and may facilitate the anti-viral drug design.
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Overall design |
Overall 10 samples are analyzed, six of which are Poly(A) RNA-seq and four are Ribozero RNA-seq samples. Poly(A) RNA-seq samples contain two treatment conditions with triplicates. Ribozero RNA-seq samples contain two treatment conditions with duplicates.
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Contributor(s) |
Hu Y, Zhao J, Zhao Y, Sun J |
Citation(s) |
34278249 |
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Submission date |
Nov 02, 2020 |
Last update date |
Jul 29, 2021 |
Contact name |
Yan Zhao |
E-mail(s) |
11962001@mail.sustech.edu.cn
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Organization name |
Southern University of Science and Technology
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Department |
BIology
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Street address |
No. 1088,Xueyuandadao
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City |
Shenzhen |
ZIP/Postal code |
518055 |
Country |
China |
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Platforms (1) |
GPL29334 |
Illumina NovaSeq 6000 (Chlorocebus sabaeus; Severe acute respiratory syndrome coronavirus 2) |
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Samples (10)
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Relations |
BioProject |
PRJNA673899 |
SRA |
SRP290847 |