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Series GSE160286 Query DataSets for GSE160286
Status Public on Mar 14, 2022
Title Transcriptional regulation of cellular mechanosignaling by PREP1 (PKNOX1)
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Mechanosignaling, initiated by the extracellular physical forces and propagated through the intracellular cytoskeletal network, triggers signaling cascades leading to processes such as embryogenesis, tissue maintenance and disease development. While signal transduction by transcription factors occurs downstream of cellular mechanosensing, nothing is known about the cell intrinsic mechanisms that can regulate mechanosignaling. Here we show that transcription factor PREP1 (PKNOX1) regulates the expression of LINC complex proteins and mechanotransduction of YAP-TAZ. PREP1 depletion upsets the nuclear membrane protein stoichiometry rendering nuclei soft. However, reduced nuclear tension is not conveyed to the cytoplasm, since these cells display fortified actomyosin network with bigger focal adhesion complexes resulting in greater traction forces at the substratum. Intriguingly, YAP-TAZ remains localized to the cytoplasm irrespective of the substrate rigidity indicating disrupted mechanotransduction. Our data demonstrate a hitherto unknown transcriptional mechanism which regulate mechanosignaling upstream of YAP-TAZ transduction.
Overall design Chip-seq analysis of Prep1 (pknox1) binding profiles in human cardiac mesenchymal stromal cells
Contributor(s) Purushothaman D, Bianchi LF, Penkov D, Poli A, Li Q, Pennacchio FA, Pramotton F, Sommariva E, Vermezovic J, Foiani M, Gauthier N, Maiuri P, Blasi F
Citation(s) 35550602
Submission date Oct 28, 2020
Last update date May 25, 2022
Contact name Francesco Blasi
Organization name IFOM
Street address Via Adamello 16
City Milan
ZIP/Postal code 20139
Country Italy
Platforms (1)
GPL17303 Ion Torrent Proton (Homo sapiens)
Samples (4)
GSM4870631 msc_cardio_bio1_IP
GSM4870632 msc_cardio_bio1_input
GSM4870633 msc_cardio_bio2_IP
BioProject PRJNA672883
SRA SRP289444

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Supplementary file Size Download File type/resource
GSE160286_RAW.tar 610.3 Mb (http)(custom) TAR (of BW)
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Raw data are available in SRA
Processed data provided as supplementary file

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