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| Status |
Public on Mar 11, 2021 |
| Title |
Immunotherapy against amyloid beta is modulated by meningeal lymphatic function V |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
A growing body of evidence points to passive immunotherapy as a promising therapeutic strategy against AD. Herein, we show that meningeal lymphatic vasculature becomes dysfunctional in AD transgenic mice and that manipulating meningeal lymphatic drainage affects the outcome of anti-Aβ immunotherapy.
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| Overall design |
Brain cells were isolated from 22-26 months old male APPswe mice, after intracisterna magna injections of 5 uL of AAV1-CMV-eGFP or AAV1-CMV-mVEGF-C (each at 1012 GC/μL) in combination with intraperitoneal injections of mIgG or mAducanumab antibodies (each at 40 mg/kg). Brain cell suspensions were obtained by enzymatic and mechanic digestion of right brain hemisphere tissue, stained for surface markers and enriched for specific populations (microglia/macrophages, blood endothelial cells and mural cells) by FACS sorting. Each individual sample used in this single cell RNA sequencing experiment resulted from brain cells pooled from 3 mice.
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| Contributor(s) |
Da Mesquita S, Papadopoulos Z, Dykstra T, Wall M, Farias FG, Brase L, Jiang H, Kodira CD, Alves de Lima K, Herz J, Louveau A, Goldman DH, Salvador AF, Onengut-Gumuscu S, Farber E, Dabhi N, Kennedy T, Milam M, Baker W, Smirnov I, Rich SS, Holtzman DM, Cruchaga C, Harari O, Kipnnis J |
| Citation(s) |
33911285, 37085492 |
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| Submission date |
Oct 27, 2020 |
| Last update date |
May 12, 2023 |
| Contact name |
Jonathan Kipnis |
| Organization name |
Washington University in Saint Louis
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| Department |
Pathology and Immunology
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| Lab |
Kipnis Lab
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| Street address |
4515 McKinley Ave
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| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63110 |
| Country |
USA |
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| Platforms (1) |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE141917 |
Immunotherapy against amyloid beta is modulated by meningeal lymphatic function |
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| Relations |
| BioProject |
PRJNA672345 |
| SRA |
SRP288787 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE160219_gfp_adu.tar.gz |
78.1 Mb |
(ftp)(http) |
TAR |
| GSE160219_gfp_igg.tar.gz |
39.5 Mb |
(ftp)(http) |
TAR |
| GSE160219_vegfc_adu.tar.gz |
37.9 Mb |
(ftp)(http) |
TAR |
| GSE160219_vegfc_igg.tar.gz |
46.6 Mb |
(ftp)(http) |
TAR |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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