GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE159763 Query DataSets for GSE159763
Status Public on Dec 11, 2020
Title (bulk RNAseq) An extracellular vesicle-related gene expression signature identifies high-risk patients in medulloblastoma
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Background: Medulloblastoma (MB) is a malignant brain tumor in childhood. It comprises four subgroups with different clinical behaviors. The aim of this study was to characterize the transcriptomic landscape of MB, both at the level of individual tumors as well as in large patient cohorts. Methods: We used a combination of single-cell transcriptomics, cell culture models and biophysical methods such as nanoparticle tracking analysis (NTA) and electron microscopy (EM), to investigate intercellular communication in the MB tumor niche. Results: Tumor cells of the SHH-MB subgroup show a differentiation blockade. These cells undergo extensive metabolic reprogramming. The gene expression profiles of individual tumor cells show a partial convergence with those of tumor-associated glial and immune cells. One possible cause is the transfer of extracellular vesicles (EVs) between cells in the tumor niche. We were able to detect EVs in co-culture models of MB tumor cells and oligodendrocytes. We also identified a gene expression signature, EVS, which shows overlap with the proteome profile of large oncosomes from prostate cancer cells. This signature is also present in MB patient samples. A high EVS expression is one common characteristic of tumors that occur in high-risk patients from otherwise very different MB subgroups or subtypes. Conclusions: With EVS, our study uncovered a novel gene expression signature that has a high prognostic significance across MB subgroups.
Overall design For bulk mRNA sequencing, total RNA was isolated using RNeasy mini Kit (Qiagen) from three different cell lines: murine oligodendrocytic cell line Oli-neu (gift of Tanja Kuhlmann, Institute of Neuropathology, University Münster), murine SHH-MB tumor cells MB1, and human Group 3 medulloblastoma cell line D341 (purchased from ATCC via LGC Standards GmBH, Wesel, Germany). MB1 cells were established from tumors of Atoh1-cre:SmoM2 mice at P12. Cells were maintained at 37°C and 5% CO2 in DMEM/F-12 medium containing B-27 and N-2 supplements (Gibco, Thermo Fisher Scientific), 20 ng/mL of recombinant murine EGF and basic fibroblast growth factor (bFGF) (PeproTech Germany), and 1% penicillin/streptomycin (Gibco). For co-culture experiments 30 mm Millicell cell culture inserts with hydrophilic PTFE membranes (0.4 µm pore size) were used (Millipore, Merck). Two biological replicates for each of the following experimental conditions were used: Oli-neu cells, MB1 cells and D341 cells grown in mono-culture (sample designations O_d1/2, M_d1/2, D_d1/2, respectively), Oli-neu cells grown in co-culture with MB1 cells (sample designations Om_d1/2 and Mo_d1/2, respectively), or Oli-neu cells grown in co-culture with D341 cells (sample designations Od_d1/2 and Do_d1/2, respectively). mRNA was extracted using the NEBNext Poly(A) mRNA Magnetic Isolation module, libraries were generated with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs), and sequencing was done using Illumina’s High Output Kit v2.5 with 75 cycles on an NextSeq500 instrument.
Contributor(s) Kerl K, Albert T, Interlandi M
Citation(s) 33175161
Submission date Oct 21, 2020
Last update date Mar 12, 2021
Contact name Kornelius Kerl
Organization name University Children's Hospital Münster
Department Department of Pediatric Hematology and Oncology
Street address Albert-Schweitzer-Campus 1
City Münster
ZIP/Postal code 48149
Country Germany
Platforms (2)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (14)
GSM4844186 1_O_d1
GSM4844187 1_O_d2
GSM4844188 2_Om_d1
This SubSeries is part of SuperSeries:
GSE160003 An extracellular vesicle-related gene expression signature identifies high-risk patients in medulloblastoma
BioProject PRJNA670439
SRA SRP287993

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE159763_D341_txi_abundance.txt.gz 469.1 Kb (ftp)(http) TXT
GSE159763_MB1_txi_abundance.txt.gz 459.6 Kb (ftp)(http) TXT
GSE159763_OLI_txi_abundance.txt.gz 619.8 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap