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Series GSE159724 Query DataSets for GSE159724
Status Public on Oct 21, 2021
Title Heat Shock Factor 1 (HSF1) supports the ESR1 action in breast cancer (ChIP-seq)
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Heat shock factor 1 (HSF1) is a key regulator of transcriptional responses to proteotoxic stress. It has been recently linked to signaling of estrogen via ESR1. To study the cooperation of HSF1 and ESR1 in the transcriptional response to estrogen, we established estrogen receptor (ER)-positive breast cancer cell lines with reduced HSF1 levels using specific shRNA or CRISPR/Cas9 approach. HSF1 deficiency led to the inhibition of the mitogenic effect of estrogen in MCF7 and T47D cells. RNA-seq analyses revealed that the stimulatory effect of E2 on the transcriptome was smaller in HSF1-deficient MCF7 cells. This could partially result from the higher basal expression of E2-dependent genes in these cells as a consequence of the enhanced binding of unliganded ESR1 to chromatin, which was revealed by ChIP-seq analyses. Thus, we postulate that some fraction of ESR1 could be released from the inhibitory complex with HSP90 and gain transcriptional competence without E2-stimulation.
 
Overall design We sequenced DNA immunoprecipitated from MCF7 cells variants with the normal level of HSF (WT and MIX) and in HSF1-deficient cells (KO#2), using mouse monoclonal anti-ERĪ± antibody. Cells were untreated (control) or stimulated using estrogen (17beta-estradiol) for indicated time. Four ChIP biological replicates were collected and pooled per each sequenced sample. Input samples were included as negative control (no DNA enrichment was observed in mock-IP samples).
 
Contributor(s) Vydra N, Janus P, Widlak W, Stokowy T
Citation(s) 34783649
Submission date Oct 21, 2020
Last update date Jan 04, 2022
Contact name Tomasz Stokowy
E-mail(s) tomasz.stokowy@uib.no
Organization name University of Bergen
Department IT Division
Lab Scientific Computing
Street address Nygardsgaten 5
City Bergen
ZIP/Postal code 5020
Country Norway
 
Platforms (1)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
Samples (10)
GSM4838749 MCF7 cells, Input
GSM4838750 MCF7 cells, WT_control
GSM4838751 MCF7 cells, WT_estrogen_30min
This SubSeries is part of SuperSeries:
GSE159803 Heat Shock Factor 1 (HSF1) supports the ESR1 action in breast cancer
Relations
BioProject PRJNA670336
SRA SRP287942

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Supplementary file Size Download File type/resource
GSE159724_RAW.tar 7.2 Mb (http)(custom) TAR (of BED, TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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