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| Status |
Public on Nov 18, 2021 |
| Title |
Transcriptional and phenotypic profile of lung-resident memory B cells induced by influenza infection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Long-lived, tissue-resident memory B cells (BRM) are established in the lungs of mice after pulmonary influenza infection. Influenza-specific BRM were localised within inducible bronchus-associated lymphoid tissues (iBALT) and displayed phenotypic and transcriptional signatures reminiscent of tissue-resident T cells, distinct from classical memory B cells in the blood or secondary lymphoid sites.
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| Overall design |
Mice were infected intranasally with 50pfu of PR8 influenza virus. Memory (Bmem) or germinal centre (GC) B cells in lung, spleen, mediastinal lymph node and blood were bulk-sorted and transcriptional gene expression profiles generated by RNAseq.
contributor: The Austin Liver Transplant Perfusionist Group
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| Contributor(s) |
Tan H, Juno JA, Esterbauer R, Kelly HG, Wragg KM, Konstandopoulos P, Alcantara S, Alvarado C, Jones R, Starkey G, Wang BZ, Yoshino O, Tiang T, Grayson ML, Opdam H, D’Costa R, Vago A, Mackay LK, Gordon CL, Masopust D, Groom JR, Kent SJ, Wheatley AK |
| Citation(s) |
35089815 |
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| Submission date |
Oct 12, 2020 |
| Last update date |
Feb 17, 2022 |
| Contact name |
Hyon Xhi Tan |
| E-mail(s) |
hxtan@unimelb.edu.au
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| Organization name |
The University of Melbourne, at the Peter Doherty Institute for Infection and Immunity
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| Department |
Department of Microbiology and Immunology
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| Street address |
792 Elizabeth St
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| City |
Melbourne |
| State/province |
Victoria |
| ZIP/Postal code |
3010 |
| Country |
Australia |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (22)
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| Relations |
| BioProject |
PRJNA668738 |
| SRA |
SRP287104 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE159383_Degust_Voom_Limma_BRM_v2.csv.gz |
696.6 Kb |
(ftp)(http) |
CSV |
| GSE159383_Raw_count_BRM_matrix_v2.txt.gz |
574.3 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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