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Series GSE159046 Query DataSets for GSE159046
Status Public on Jan 27, 2021
Title Stronger induction of trained immunity by mucosal BCG or MTBVAC vaccination
Organism Macaca mulatta
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Vaccination against tuberculosis by intradermal Bacillus Calmette-Guérin (BCG) injection saves many lives, supposedly by inducing adaptive immune memory in lymphocytes. Epidemiologically, BCG vaccination is also associated with reduced childhood mortality unrelated to TB, which is attributed to innate immune memory, also termed trained immunity. We recently demonstrated improved protection against tuberculosis infection in highly susceptible rhesus macaques by mucosal BCG vaccination, correlating with a unique local but no peripheral immune profile. Here, we investigated local and peripheral innate immune function after intradermal versus mucosal vaccination with M. bovis BCG or the live attenuated, M. tuberculosis-derived candidate, MTBVAC. The results demonstrate an augmented frequency of trained immunity in monocytes after respiratory mucosal administration of live attenuated mycobacterial vaccines compared to intradermal immunization, with MTBVAC being equally potent as BCG. These results provide further support to strategies for improving TB vaccination and, more broadly, modulating innate immunity via mucosal surfaces.
Overall design ChIP-seq using primate cells pre-treatment or treated with BCG
Contributor(s) Vierboom MP, Domínguez-Andrés J, van der Sande M, Martens JH, Verreck FA
Citation(s) 33521699
Submission date Oct 05, 2020
Last update date Nov 11, 2021
Contact name Joost Martens
Phone 0243780645
Organization name Radboud University
Department RIMLS
Lab Molecular Biology
Street address Geert Grooteplein 28
City Nijmegen
State/province Nederland
ZIP/Postal code 6525GA
Country Netherlands
Platforms (1)
GPL21120 Illumina NextSeq 500 (Macaca mulatta)
Samples (6)
GSM4818172 H3K27ac-NHP-LvE-1-17867
GSM4818173 H3K27ac-NHP-LvE-2-17868
GSM4818174 H3K27ac-NHP-LvE-3-17869
BioProject PRJNA667474
SRA SRP286416

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Supplementary file Size Download File type/resource
GSE159046_RAW.tar 293.3 Mb (http)(custom) TAR (of BIGWIG)
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Raw data are available in SRA
Processed data provided as supplementary file

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