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Series GSE158890 Query DataSets for GSE158890
Status Public on Dec 15, 2021
Title Transcriptome analysis using Quant-RNAseq after NPM1 or HIF-1α silencing under normoxia or Hypoxia
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Hypoxia inducible factor-1 (HIF-1) is a central transcriptional regulator of genes associated with adaptive responses to hypoxia. NPM1 is a histone chaperone found to associate with HIF-1α in a phosphorylation dependent manner and increase its activty. The aim of this study was to find if HIF-1α and NPM1 regualate gene expression under hypoxia. Transcriptome analysis using Quant-RNA-seq after HIF-1α or NPM1 silencing under hypoxia reveals a significant number of genes, the hypoxic expression of which depends on both proteins.
Overall design Examinaton of genes expressed after silencing with siRNA NPM1 or HIF-1α in HeLa cells and subsequent incubaton in normaxia or hypoxia.
Contributor(s) Koukoulas K, Giakountis A, Simos G, Mylonis I
Citation(s) 34388291
Submission date Oct 01, 2020
Last update date Dec 15, 2021
Contact name Antonis Giakountis
Organization name University of Thessaly
Department Biochemistry and Biotechnology
Lab Laboratory of Molecular Biology and Genomics
Street address Biopolis
City Larissa
ZIP/Postal code 41500
Country Greece
Platforms (1)
GPL17303 Ion Torrent Proton (Homo sapiens)
Samples (10)
GSM4815223 Nt Normoxia rep1
GSM4815224 Nt Normoxia rep2
GSM4815225 Nt Hypoxia rep1
BioProject PRJNA666907
SRA SRP286039

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Supplementary file Size Download File type/resource
GSE158890_Normalized_counts_table_Control_vs_HIF1_NPM1_Hyp.txt.gz 1.4 Mb (ftp)(http) TXT
GSE158890_Normalized_counts_table_Control_vs_NPM1_Norm.txt.gz 1.3 Mb (ftp)(http) TXT
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