NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE158547 Query DataSets for GSE158547
Status Public on May 04, 2021
Title Multi-tissue Single-Cell Analysis Deconstructs the Complexities of Mouse NK-ILC1 Programs
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Natural killer (NK) cells and type 1 innate lymphoid cells (ILC1s) are a heterogenous group of T-bet+ innate cells that produce IFN-γ and are broadly defined as lineage–NK1.1+NKp46+ cells in mice. ILC1s definition primarily stems from studies on liver-resident and small intestinal populations. However, ILC1s in many anatomical sites, including visceral adipose tissue, salivary glands, and uterus, exhibit non-uniform programs that do not adequately overlap with those of liver or gut ILC1s or NK cells. Here, we performed single-cell RNA sequencing on murine NK1.1+NKp46+ cells from blood, spleen, lymph nodes, liver, salivary gland, uterus, visceral adipose tissue, small intestines, and several solid tumors. By including cells from an array of niches we defined tissue-specific ILC1 subsets. Moreover, we found a significant heterogeneity of circulating NK cells, due to a spectrum of proliferating, effector and migration programs, which was reduced in tumor-bearing mice, demonstrating repertoire reshaping in response to diseased conditions.
 
Overall design NK/ILC1 cells (NK1.1+,NCR1+<Lin-) cells are collected from different tissues of WT or tumor-bearing C57BL/6 animals. Cells are sorted and single cell library of tissue-specific NK cells is sequenced.
Web link https://doi.org/10.1016/j.immuni.2021.03.024
 
Contributor(s) McFarland1 AP, Jelinski A, Wang S, Cortez VS, Landsberger T, Miller H, Ricci B, David E, Faccio R, Amit I, Colonna M
Citation(s) 33945787
Submission date Sep 24, 2020
Last update date Aug 04, 2021
Contact name Ido Amit
E-mail(s) ido.amit@weizmann.ac.il
Phone 972-8-9343338
Organization name Weizmann Institute of Science
Department Immunology
Street address 234 Herzl st.
City Rehovot
ZIP/Postal code 760001
Country Israel
 
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (130)
GSM4802543 AB3875
GSM4802544 AB3876
GSM4802545 AB3911
Relations
BioProject PRJNA665541
SRA SRP285322

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE158547_RAW.tar 52.8 Mb (http)(custom) TAR (of TXT)
GSE158547_metadata_s.txt.gz 852.4 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap