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Status |
Public on May 04, 2021 |
Title |
Multi-tissue Single-Cell Analysis Deconstructs the Complexities of Mouse NK-ILC1 Programs |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Natural killer (NK) cells and type 1 innate lymphoid cells (ILC1s) are a heterogenous group of T-bet+ innate cells that produce IFN-γ and are broadly defined as lineage–NK1.1+NKp46+ cells in mice. ILC1s definition primarily stems from studies on liver-resident and small intestinal populations. However, ILC1s in many anatomical sites, including visceral adipose tissue, salivary glands, and uterus, exhibit non-uniform programs that do not adequately overlap with those of liver or gut ILC1s or NK cells. Here, we performed single-cell RNA sequencing on murine NK1.1+NKp46+ cells from blood, spleen, lymph nodes, liver, salivary gland, uterus, visceral adipose tissue, small intestines, and several solid tumors. By including cells from an array of niches we defined tissue-specific ILC1 subsets. Moreover, we found a significant heterogeneity of circulating NK cells, due to a spectrum of proliferating, effector and migration programs, which was reduced in tumor-bearing mice, demonstrating repertoire reshaping in response to diseased conditions.
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Overall design |
NK/ILC1 cells (NK1.1+,NCR1+<Lin-) cells are collected from different tissues of WT or tumor-bearing C57BL/6 animals. Cells are sorted and single cell library of tissue-specific NK cells is sequenced.
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Web link |
https://doi.org/10.1016/j.immuni.2021.03.024
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Contributor(s) |
McFarland1 AP, Jelinski A, Wang S, Cortez VS, Landsberger T, Miller H, Ricci B, David E, Faccio R, Amit I, Colonna M |
Citation(s) |
33945787 |
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Submission date |
Sep 24, 2020 |
Last update date |
Aug 04, 2021 |
Contact name |
Ido Amit |
E-mail(s) |
ido.amit@weizmann.ac.il
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Phone |
972-8-9343338
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Organization name |
Weizmann Institute of Science
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Department |
Immunology
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Street address |
234 Herzl st.
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City |
Rehovot |
ZIP/Postal code |
760001 |
Country |
Israel |
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Platforms (1) |
GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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Samples (130)
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Relations |
BioProject |
PRJNA665541 |
SRA |
SRP285322 |
Supplementary file |
Size |
Download |
File type/resource |
GSE158547_RAW.tar |
52.8 Mb |
(http)(custom) |
TAR (of TXT) |
GSE158547_metadata_s.txt.gz |
852.4 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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