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Series GSE158200 Query DataSets for GSE158200
Status Public on Dec 22, 2021
Title Single cell RNA-seq analysis of novel splenic DC
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Other
Summary To understand the cellular diversity within CD11b+ splenocytes between control and GM-CSF-treated mice, we performed the CITE-seq in control and GM-CSF treated mice.
 
Overall design FACS sorted CD11b+ splenocytes were loaded into the Chromium system (10x Genomics, Pleasanton, CA, USA) targeting 7,000 cells per sample. The cDNA libraries for mRNA were generated using Chromium Single Cell 3' v3 Reagent Kits according to the manufacturer’s instruction. Following the CITE-seq protocol, ADT PCR additive primers were added to cDNA PCR and the ADT libraries were generated separately from the mRNAs.
 
Contributor(s) Park C, Ryu S, Eum H, Lee H
Citation(s) 35069539
Submission date Sep 18, 2020
Last update date Jan 31, 2022
Contact name Chae Gyu Park
E-mail(s) chaegyu@gmail.com
Organization name YONSEI UNIVERSITY
Street address 50 Yonsei-ro, Seadaemun-gu
City Seoul
ZIP/Postal code 03722
Country South Korea
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (4)
GSM4795134 CD11b+ Splenocytes in steady state_RNA data
GSM4795135 CD11b+ Splenocytes in steady state_ADT data
GSM4795136 CD11b+ Splenocytes in GM-conditioned state_RNA data
Relations
BioProject PRJNA664351
SRA SRP283450

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE158200_MoMacDC_ADT_count_matrix.txt.gz 65.6 Kb (ftp)(http) TXT
GSE158200_MoMacDC_RNA_count_matrix.txt.gz 13.6 Mb (ftp)(http) TXT
GSE158200_MoMacDC_cell_metadata.txt.gz 259.0 Kb (ftp)(http) TXT
GSE158200_Total_ADT_count_matrix.txt.gz 112.5 Kb (ftp)(http) TXT
GSE158200_Total_RNA_count_matrix.txt.gz 21.7 Mb (ftp)(http) TXT
GSE158200_Total_cell_metadata.txt.gz 271.7 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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