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| Status |
Public on Dec 22, 2021 |
| Title |
Single cell RNA-seq analysis of novel splenic DC |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
|
| Summary |
To understand the cellular diversity within CD11b+ splenocytes between control and GM-CSF-treated mice, we performed the CITE-seq in control and GM-CSF treated mice.
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| Overall design |
FACS sorted CD11b+ splenocytes were loaded into the Chromium system (10x Genomics, Pleasanton, CA, USA) targeting 7,000 cells per sample. The cDNA libraries for mRNA were generated using Chromium Single Cell 3' v3 Reagent Kits according to the manufacturer’s instruction. Following the CITE-seq protocol, ADT PCR additive primers were added to cDNA PCR and the ADT libraries were generated separately from the mRNAs.
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| |
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| Contributor(s) |
Park C, Ryu S, Eum H, Lee H |
| Citation(s) |
35069539 |
| |
| Submission date |
Sep 18, 2020 |
| Last update date |
Jan 31, 2022 |
| Contact name |
Chae Gyu Park |
| E-mail(s) |
chaegyu@gmail.com
|
| Organization name |
YONSEI UNIVERSITY
|
| Street address |
50 Yonsei-ro, Seadaemun-gu
|
| City |
Seoul |
| ZIP/Postal code |
03722 |
| Country |
South Korea |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (4)
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| GSM4795134 |
CD11b+ Splenocytes in steady state_RNA data |
| GSM4795135 |
CD11b+ Splenocytes in steady state_ADT data |
| GSM4795136 |
CD11b+ Splenocytes in GM-conditioned state_RNA data |
| GSM4795137 |
CD11b+ Splenocytes in GM-conditioned state_ADT data |
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| Relations |
| BioProject |
PRJNA664351 |
| SRA |
SRP283450 |
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