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Series GSE158072 Query DataSets for GSE158072
Status Public on Apr 13, 2021
Title microRNA from Ionizing radiation treatment of chondrocyte-like cells derived from human induced pluripotent stem cells: gene and microRNA expression profile and high-throughput screening of secreted cytokines, chemokines, and growth factors
Platform organism synthetic construct
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary Human induced pluripotent stem cells (hiPSCs) possess the ability to differentiate into chondrocytes and produce cartilaginous matrix without the need to biopsy healthy cartilage. However, this strategy is not without risk given that those transplanted cells can be subjected to IR during Head and Neck (H&N) (re-)treatment. The main aim of this study was to investigate the microRNA expression profile after ionizing radiation (IR) treatment of chondrocyte-like cells differentiated from hiPSCs. The differentiated hiPSC into chondrocyte were irradiated in accordance with conventional therapy, hypofractionation and hyperfractionation protocols (1, 2, 3 Gy) intended for larynx region. Then, to fulfill specific objective we performed following high-throughput analysis: gene expression profile based on microarrays. Our findings allowed for the selection of crucial biological processes activated in hiPSC-derived chondrocytes after IR. Moreover, we observed decrease of expression of genes involved in e.g. cell cycle and cell division. Next, we have selected crucial microRNAs which are responsible for controlling genes of aforementioned biological processes. Microarray data were confirmed by RT-qPCR technique. We have proved that IR have a great impact on DDR mechanisms of hiPSCs differentiated towards chondrogenic lineage.
Overall design To irradiate the chondrocyte-like cells the specially modified and redesigned the Alderson Radiation Therapy phantom (ART) was used to obtain similar condition to those in patient’s larynx region treatment. Eppendorf tube with the one million of chondrocyte-like cells was used to irradiate the cells and was delineated as the quasi-Gross tumor volume (GTV), defining 1 cm geometrical margins around GTV to create the quasi-Clinical Target Volume (CTV) was done. The miRNA-enriched fraction from hiPSCs after chondrogenic differentiation were purified with miRNeasy Kit and RNeasy MinElute according to the manufacturer’s instructions (217004, Quiagen, Hilden, Germany).
Contributor(s) Stelcer E, Kulcenty K, Rucinski M
Citation(s) 33820914
Submission date Sep 16, 2020
Last update date Apr 13, 2021
Contact name Marcin Rucinski
Organization name Poznan University of Medical Science
Department Histology and Embriology
Street address Swiecicki 6
City Poznan
ZIP/Postal code 60-781
Country Poland
Platforms (1)
GPL19117 [miRNA-4] Affymetrix Multispecies miRNA-4 Array
Samples (12)
GSM4790084 Control 24h, biological rep 1 [miRNA]
GSM4790085 Control 24h, biological rep 2 [miRNA]
GSM4790086 Control 24h, biological rep 3 [miRNA]
This SubSeries is part of SuperSeries:
GSE158073 Ionizing radiation treatment of chondrocyte-like cells derived from human induced pluripotent stem cells: gene and microRNA expression profile
BioProject PRJNA663888

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE158072_RAW.tar 10.8 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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