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Series GSE158025 Query DataSets for GSE158025
Status Public on Nov 13, 2020
Title A ubiquitin ligase mediates target-directed microRNA decay independently of tailing and trimming [small RNA-seq]
Organisms Homo sapiens; Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary MicroRNAs (miRNAs) act in concert with Argonaute (AGO) proteins to broadly repress mRNA targets. After AGO loading, miRNAs generally exhibit slow turnover. An important exception occurs when miRNAs encounter targets with extensive complementarity, which can trigger a process termed target-directed microRNA degradation (TDMD). Prevailing models of TDMD invoke miRNA tailing and trimming as an essential step in the decay mechanism. Here, a genome-wide screen revealed a novel cullin-RING ubiquitin ligase, which we named the DECAY complex, that mediates TDMD. The DECAY complex interacts with AGO proteins, mediates TDMD induced by multiple transcripts, and does not require tailing and trimming to elicit miRNA turnover. Based upon these findings, we propose a model in which the DECAY complex mediates TDMD by promoting proteasomal decay of miRNA-containing complexes.
Overall design Total RNA was isolated from biological triplicates of each cell line using the miRNeasy Mini RNA isolation kit (QIAGEN). Library preparation and next generation sequencing were performed by DNA Link using NEB Next Small RNA Library Prep for Illumina (New England BioLabs).
Contributor(s) Han J, LaVigne CA, Jones BT, Zhang H, Gillett F, Mendell JT
Citation(s) 33184234
Submission date Sep 16, 2020
Last update date Feb 12, 2021
Contact name Joshua Mendell
Organization name UT Southwestern Medical Center
Department Molecular Biology
Street address 6000 Harry Hines Blvd., NA6.200A
City Dallas
State/province TX
ZIP/Postal code 75390-9148
Country USA
Platforms (2)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (18)
GSM4785414 smallRNAseq_HEK293T_nontarget control-1
GSM4785415 smallRNAseq_HEK293T_nontarget control-2
GSM4785416 smallRNAseq_HEK293T_nontarget control-3
This SubSeries is part of SuperSeries:
GSE151517 A ubiquitin ligase mediates target-directed microRNA decay independently of tailing and trimming
BioProject PRJNA663788
SRA SRP282564

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Supplementary file Size Download File type/resource
GSE158025_RPM.xlsx 212.6 Kb (ftp)(http) XLSX
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Processed data are available on Series record

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